FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14000612
Sequences flagged as poor quality0
Sequence length50-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG201250.010.199647120
GAGTGTA223700.09.310187120
TAGATCT145200.09.300597120
AGAGTGT249300.08.907881120
GTAGGGA435600.08.597886120
TGTAGGG474400.08.552576120
TAGGGAA409500.08.515637120
GTGTAGA216900.08.495204120
GTAGATC237300.08.017823120
AAGAGTG287000.07.821403120
GTGTAGG519150.07.8037953120
TCGTGTA586150.07.536401120
GTCGTGT626750.07.5078683120
AGCGTCG808800.07.294698120
CGTGTAG560150.07.2808156118-119
CGTCGTG735700.07.1873717120
AAAGAGT341900.07.0570292120
AGGGAAA478750.06.9829893120
GGGAAAG450300.06.7577324120
TGTAGAT242800.06.748526120
Produced by FastQC (version 0.11.5)