FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21172059
Sequences flagged as poor quality0
Sequence length50-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT198050.08.027709120
TGTAGGG535900.07.870316120
AGTGTAG259750.07.853152120
GAGTGTA286200.07.8191547120
TAGGGAA467100.07.603831120
GTAGATC282350.07.4583015120
GTGTAGG590500.07.3864393120
AGAGTGT324000.07.277264120
GTAGGGA487000.07.225363118-119
CGTGTAG621200.07.185584120
AAGAGTG367350.07.0717754120
GTCGTGT700400.07.041194120
TCGTGTA664450.07.0158343120
GTGTAGA282700.06.8336177120
AGCGTCG913800.06.5458283120
AGATCTC232800.06.4686165120
CGTCGTG808400.06.456744120
GCGTCGT861150.06.256308120
GGGAAAG543350.06.216549120
GAGCGTC973050.06.1349154120
Produced by FastQC (version 0.11.5)