FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12740305
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT146400.010.285766120
AGTGTAG206300.09.422144120
GTGTAGA215150.09.20188120
GTAGGGA426200.08.994798120
GAGTGTA224200.08.616368118-119
TGTAGGG468300.08.50644120
AAGAGTG293000.08.435942120
TAGGGAA402950.08.307819120
GTAGATC220950.08.064295120
GTGTAGG511100.08.040604120
CGTGTAG538950.07.903398120
CGTCGTG679950.07.8791337120
TCGTGTA580300.07.8674874120
AGAGTGT253600.07.854034120
GTCGTGT612200.07.7319255120
AGCGTCG763650.07.510476120
GGGAAAG432550.07.5035176120
GAGCGTC826800.07.3504333120
AGATCTC166100.07.259896120
GATCTCG179450.07.1209855120
Produced by FastQC (version 0.11.5)