FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13929907
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC118900.061.22647120
GAGCACA183900.041.897884120
AGAGCAC239450.032.689182120
AAGAGCA384250.020.957466120
GAAGAGC427050.018.57043120
GGAAGAG555700.014.850852120
TCGGAAG623950.013.680713120
CGGAAGA664100.012.756599120
GATCGGA716850.012.258256120
TAGATCG205550.011.909917120
ATCGGAA745400.011.526563118-119
AGATCGG791800.011.48846120
CTAGATC48700.010.582874120
TTAGATC77800.09.558132118-119
GAGATCG264400.08.985361118-119
AAGATCG311800.08.60022118-119
ACACGTC49000.08.2829895120
CAGATCG256450.08.2220335118-119
TGAGATC103000.07.2197804112-113
GCTAGAT22100.07.1375146116-117
Produced by FastQC (version 0.11.5)