FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14213593
Sequences flagged as poor quality0
Sequence length58-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG72550.09.84129120
AGTGTAG342700.08.49122120
AGATCTC240000.08.474829120
TAGATCT239700.08.385313120
ATCTCGG233600.08.270381120
GATCTCG242200.08.249214120
GAGTGTA369150.08.142867120
GTGTAGA347200.07.9664288120
AGAGTGT400450.07.9259233120
GTAGATC348100.07.91136120
TCTCGGT209450.07.8203244120
AAGAGTG454250.07.5947833120
CTCGGTG196800.07.560823120
TGTAGAT382300.07.4390335120
CGTGTAG799050.07.2985196120
TAGGGAA639550.07.2893534120
GTGTAGG757150.07.2190313120
GTAGGGA660100.07.2078533120
GGTGGTC175650.07.173211120
TGTAGGG694700.07.151144120
Produced by FastQC (version 0.11.5)