FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14213593
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC121000.060.46232120
GAGCACA180000.041.38182120
AGAGCAC239900.031.41831120
AAGAGCA383950.020.338976120
GAAGAGC436300.017.6522118-119
GGAAGAG565550.014.054036120
TCGGAAG635750.013.108888120
GATCGGA720850.012.482363120
CGGAAGA687850.012.115978120
ATCGGAA749950.011.787228120
AGATCGG792100.011.327634120
TAGATCG208950.010.561361120
CTAGATC49600.010.198708118-119
GAGATCG289900.09.09546120
CAGATCG295200.08.71796120
AAGATCG290950.08.497579120
TTAGATC86700.08.277649114-115
CCTAGAT19000.07.8208275116-117
TCTAGAT28750.07.037995120
AGAGATC103900.06.7552934120
Produced by FastQC (version 0.11.5)