FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15474731
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC183150.071.28181120
GAGCACA288850.043.720036120
AGAGCAC380500.033.803448118-119
AAGAGCA611700.022.54161120
GAAGAGC655450.021.169249120
GGAAGAG801650.017.416658120
TCGGAAG955100.015.372389120
CGGAAGA939300.015.354127120
GATCGGA1157650.013.126228120
ATCGGAA1153450.013.02951120
AGATCGG1287950.012.367732120
TAGATCG285750.011.900271120
CTAGATC75500.010.509279120
TTAGATC116100.09.906959118-119
AAGATCG506750.09.282965118-119
GAGATCG457850.09.276607120
CACACGT48200.09.268312120
CAGATCG432350.08.721287118-119
CACGTCT53300.08.631669120
ATAGATC131650.08.052842120
Produced by FastQC (version 0.11.5)