FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12764100
Sequences flagged as poor quality0
Sequence length52-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCTC401650.010.502771120
TGGTCGC190300.09.585862120
TAGATCT410600.09.557738120
GTGGTCG210200.09.50622120
TCTCGGT326550.09.187908120
GAGTGTA622900.09.151696120
GGTGGTC236200.09.1203375120
GTGTAGA550100.09.119289120
ATCTCGG363250.09.036037120
AGTGTAG575650.08.985559120
CTCGGTG312350.08.875581120
GATCTCG398950.08.663639120
TGTAGAT576750.08.656295120
GTAGATC555500.08.566147120
TCGGTGG277600.08.559966120
AAGAGTG746500.08.472414120
AGAGTGT675250.08.379989120
CGGTGGT277000.07.950284120
GGTCGCC201650.07.8560104120
TAGGGAA1083100.07.4848504120
Produced by FastQC (version 0.11.5)