FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12764100
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC134600.072.762085120
GAGCACA218900.046.317112120
AGAGCAC291500.035.12297120
AAGAGCA457900.022.489765118-119
GAAGAGC505650.020.379114120
GGAAGAG609400.017.072906120
CGGAAGA758550.014.175183118-119
TCGGAAG766150.014.064881120
GATCGGA905400.012.22417120
ATCGGAA898400.012.061024116-117
AGATCGG1007600.011.03038120
CACACGT56400.010.705973120
TAGATCG244250.010.486162120
CACGTCT58450.09.308788120
CTAGATC53000.09.076799114-115
TTAGATC91250.08.653223120
GAGATCG444500.08.650585118-119
ACACGTC63750.08.430798120
ACGTCTG67250.08.386687118-119
CAGATCG354450.07.806294120
Produced by FastQC (version 0.11.5)