FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16182544
Sequences flagged as poor quality0
Sequence length50-126
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT114850.022.154
CTTCCGA120450.022.0169431
CGATCTA31300.020.510475
TCCGATC130100.020.3376833
TTCCGAT175500.015.3158972
AAAAAAA1336400.012.924547120
CGTATCA603900.010.673338120
GCCGTAT744450.010.351195120
GCGTCGG91300.010.188774120
CGCCGTA803200.010.139514120
CCGTATC689150.010.101951120
TGGTCGC1097650.010.076796120
GGTCGCC1018450.09.91168120
GTCGCCG973050.09.800533120
TCGCCGT909150.09.796238120
GTATCAT661450.09.118639120
GTGGTCG1186650.09.108609120
GGTGGTC1282950.08.845916120
CGGTGGT1407650.08.646379120
TCGGTGG1510800.08.516846120
Produced by FastQC (version 0.11.5)