FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16182544
Sequences flagged as poor quality0
Sequence length50-126
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC224450.098.60279120
GAGCACA388150.058.817326120
AGAGCAC525350.043.260212120
AAGAGCA784700.029.283276118-119
CTTCCGA122800.026.2868791
GAAGAGC871100.025.764757116-117
CCGATCT125450.025.103464
CGATCTA33550.024.114045
TCCGATC137200.023.1514423
GGAAGAG1071300.021.356674116-117
CGGAAGA1231450.018.322115114-115
TTCCGAT180400.018.0287882
TCGGAAG1288850.017.702822114-115
CACGTCT67750.015.114593120
ATCGGAA1516350.015.070389112-113
CACACGT58600.014.7653885120
ACACGTC67100.014.669496120
GATCGGA1591800.014.475512112-113
ACGTCTG78700.013.515933120
AGATCGG1728250.013.233896110-111
Produced by FastQC (version 0.11.5)