FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14935186
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1123600.09.9536295120
GCGTCGG132700.09.3141575120
CGTATCA418900.08.780058120
GTGGTCG755600.08.202678120
GGTCGCC690400.08.082197120
TGGTCGC696500.08.028642120
CGCCGTA601050.07.975969120
GCCGTAT545450.07.754994120
GTATCAT437450.07.749386120
TCTCGGG51350.07.7117114120
GGTGGTC812150.07.675852120
CGGTGGT883300.07.675691120
CCGTATC509100.07.589795120
TCGGTGG920800.07.565091120
CTCGGTG996650.07.5492215120
GTCGCCG720200.07.489517120
CATTAAA407000.07.282489120
TCTCGGT1042100.07.2660303120
AGATCTC1239100.07.190637120
TCATTAA393200.07.11082120
Produced by FastQC (version 0.11.5)