FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14935186
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC166500.088.85502120
GAGCACA276750.052.812492120
AGAGCAC380900.039.05511118-119
AAGAGCA579100.025.731447118-119
GAAGAGC669800.022.34357120
GGAAGAG799700.018.736015118-119
CGGAAGA939450.016.04332114-115
TCGGAAG950100.015.792162116-117
ATCGGAA1105200.013.915898120
GATCGGA1159200.013.754247120
AGATCGG1284000.012.244686118-119
TAGATCG276950.012.143120
CTAGATC86800.010.61122118-119
TTAGATC118450.09.252705118-119
AAGATCG520850.09.184248116-117
GAGATCG489100.08.97056118-119
CAGATCG440250.08.936091118-119
CACACGT34550.08.473099120
CACGTCT42700.08.360815120
TAAGATC216300.08.186563120
Produced by FastQC (version 0.11.5)