FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13223144
Sequences flagged as poor quality0
Sequence length56-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC169450.08.816383120
TGGTCGC181200.08.6420145120
TAGATCT341750.08.47952120
ATCTCGG299600.08.450908120
AGATCTC333800.08.411863120
GCGTCGG64250.08.310928120
GTGGTCG191500.08.083205120
GTGTAGA448250.08.071044120
TCTCGGT280700.08.058082120
AGTGTAG461550.07.981459120
CTCGGTG283850.07.820698120
GTAGATC437500.07.789382120
AGAGTGT537450.07.7585297120
GATCTCG324300.07.696248120
TCGGTGG260500.07.600443120
GAGTGTA492700.07.5864425120
AAGAGTG591950.07.4496355120
TAGGGAA818100.07.194412120
GGTGGTC220600.07.18011120
GAAAGAG774350.07.0042853120
Produced by FastQC (version 0.11.5)