FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13223144
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC141800.057.92548120
GAGCACA211250.038.546593120
AGAGCAC271800.031.381536120
AAGAGCA427550.019.99492120
GAAGAGC448900.019.109123118-119
GGAAGAG555550.016.037426120
TCGGAAG625000.014.791341120
CGGAAGA627700.013.783932118-119
ATCGGAA766600.012.403756120
GATCGGA761200.012.347874120
AGATCGG847250.011.215785118-119
TAGATCG197050.010.7888120
CTAGATC55800.010.160093118-119
CACACGT38350.08.903201120
AAGATCG409000.08.852149120
TTAGATC100050.08.628263120
GAGATCG275800.08.491011118-119
CACGTCT44050.08.336133120
ACACGTC40800.08.13198118-119
CAGATCG261200.07.2265353120
Produced by FastQC (version 0.11.5)