FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15208838
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC134600.061.49707120
GAGCACA211650.039.471527120
AGAGCAC290000.031.93482120
AAGAGCA449150.020.604946120
GAAGAGC531500.017.724876118-119
GGAAGAG632100.016.106344120
TCGGAAG725650.014.179562120
CGGAAGA751100.013.852169120
CTAGATC67000.013.05991120
GATCGGA878200.012.341924120
ATCGGAA875000.012.255671120
AGATCGG973650.011.289441120
TAGATCG277550.011.252839120
TTAGATC109200.09.182723120
CCTAGAT22550.08.780305118-119
TCTAGAT37750.08.121172118-119
GCTAGAT29050.07.914998120
GAGATCG386700.07.8288617120
TACCTAG8200.00198616727.7889805120
AAGATCG361700.07.787258118-119
Produced by FastQC (version 0.11.5)