FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12008361
Sequences flagged as poor quality0
Sequence length50-126
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC118450.072.12395120
GAGCACA185050.044.869534120
AGAGCAC243750.034.5275118-119
AAGAGCA421750.021.280462120
GAAGAGC418600.020.70289118-119
GGAAGAG507850.016.997072118-119
CGGAAGA570200.015.186712116-117
TCGGAAG571900.014.979787116-117
ATCGGAA681650.012.904702114-115
GATCGGA696050.012.416185114-115
CACGTCT35500.012.167706120
AGATCGG764900.011.49556112-113
CACACGT32550.011.264558118-119
TAGATCG178800.010.770963116-117
ACACGTC38000.010.1041765120
CTAGATC51400.09.73262110-111
AAGATCG344950.09.441347120
ACGTCTG44650.09.213535120
GAGATCG240700.08.684306118-119
TAAGATC157150.08.350729110-111
Produced by FastQC (version 0.11.5)