FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6170650
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC78050.087.558426120
GAGCACA126150.052.887432120
AGAGCAC173450.042.630234120
AAGAGCA270450.026.236439118-119
GAAGAGC299750.023.364449118-119
GGAAGAG354050.020.051773116-117
CGGAAGA415150.016.86979116-117
TCGGAAG424150.016.67695114-115
ATCGGAA498250.014.322511120
GATCGGA507750.013.938383120
AGATCGG545650.012.821628118-119
TAGATCG133300.012.443527110-111
ACACGTC21350.011.394813120
CTAGATC30750.011.148051114-115
TTAGATC42250.011.079959108-109
CACACGT17300.011.079454120
CACGTCT19150.010.779047120
GCTAGAT13450.010.414116120
GAGATCG181800.010.178199120
CAGATCG230750.09.808166110-111
Produced by FastQC (version 0.11.5)