FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14538893
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC151300.052.046486120
GAGCACA200800.038.32212120
AGAGCAC265850.030.15125120
AAGAGCA428800.019.979458120
GAAGAGC460000.018.63827120
GGAAGAG566400.015.476661120
TCGGAAG648000.013.8145485118-119
CGGAAGA673800.013.771128120
ATCGGAA787250.012.226439120
GATCGGA772700.012.000224120
AGATCGG852350.011.1572120
TAGATCG211150.010.629405120
CTAGATC54100.010.171834116-117
CACGTCT41300.09.626616120
TTAGATC90900.09.0297985120
AAGATCG360050.08.059506118-119
ACACGTC40700.07.782243118-119
GAGATCG325000.07.7474613116-117
CACACGT39000.06.972978118-119
CAGATCG306300.06.925626114-115
Produced by FastQC (version 0.11.5)