FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12946331
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC130900.064.624016120
GAGCACA201800.042.466057120
AGAGCAC266950.033.05055120
AAGAGCA418850.022.567944120
GAAGAGC471200.019.508303118-119
GGAAGAG585800.015.730653118-119
TCGGAAG680500.014.07191120
CGGAAGA695500.013.675073120
ATCGGAA811000.012.78419120
GATCGGA816350.012.302775120
AGATCGG903750.011.579928120
CTAGATC54250.011.120995116-117
TAGATCG207950.010.934123118-119
TTAGATC84850.09.181604120
GAGATCG363250.08.08998118-119
AAGATCG350750.07.7369137120
CCTAGAT20300.07.6754594120
CAGATCG362750.07.636264116-117
CACACGT34250.07.5820837120
GCTAGAT24000.07.033172120
Produced by FastQC (version 0.11.5)