FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004833295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11773810
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC159450.081.53148120
GAGCACA245450.051.568424120
AGAGCAC323950.039.457005120
AAGAGCA481250.026.6735120
GAAGAGC536650.023.570053116-117
GGAAGAG643550.020.539652120
CGGAAGA755100.017.039694118-119
TCGGAAG759200.016.840052118-119
CACGTCT46650.015.3613615120
ATCGGAA903350.014.398426116-117
GATCGGA922850.014.0857525116-117
AGATCGG1009700.012.939745114-115
CACACGT41300.012.906324118-119
TAGATCG184050.012.282466110-111
CTAGATC55350.012.231309108-109
ACACGTC50700.011.829769120
ACGTCTG56850.010.687045120
CGTCTGA68150.010.629446120
AAGATCG436200.010.462843114-115
TTAGATC71400.010.081353110-111
Produced by FastQC (version 0.11.5)