FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72940
Sequences flagged as poor quality0
Sequence length301
%GC54

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG18432.526734302166164No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG18122.4842336166712364No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG15422.1140663559089665No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG13181.8069646284617495No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG12081.656155744447491No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG11581.5876062517137375No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC11481.5738963531669865No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG11451.5697833836029615No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG10691.4655881546476557No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG10171.3942966822045517No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC10061.3792157938031258No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG10031.3751028242391006No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG9811.344941047436249No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCG9541.307924321360022No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC9461.2969564025226212No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG9441.2942144228132713No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCG9321.2777625445571703No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCG9071.2434877981902934No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG9001.2338908692075679No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCG8801.2064710721140663No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG8541.1708253358925143No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG8381.1488894982177134No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG8141.1159857417055112No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG8101.110501782286811No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG7641.0474362489717577No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC7541.0337263504250067No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC7421.017274472168906No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG7311.0021935837674802No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG7150.9802577460926789No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG7110.9747737866739787No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCG6040.8280778722237455No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC5320.72936660268714No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG5260.7211406635590897No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG5200.7129147244310392No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG5140.7046887853029888No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCG4570.6265423635865095No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG4490.6155744447491088No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG4460.6114614751850835No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCACAATGGGCGAAAGCCTGAT4200.5758157389635317No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCACAATGGGCGAAAGCCTGAT4190.5744447491088567No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT3870.5305730737592541No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT3760.5154921853578284No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3690.5058952563751028No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3670.5031532766657526No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT3390.46476556073485054No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT3310.4537976418974499No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCACAATGGGCGAAAGCCTGAT3240.44420071291472446No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG3190.43734576364134903No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3140.4304908143679737No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG3100.42500685494927337No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG3050.418151905675898No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCACAATGGGCGAAAGCCTGAT2990.4099259665478475No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG2960.40581299698382234No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2890.39621606800109677No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2860.3921030984370716No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2830.38799012887304635No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCACAATGGGCGAAAGCCTGAT2820.38661913901837125No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC2760.3783931998903208No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG2650.363312311488895No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT2620.3591993419248698No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC2550.3496024129421442No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2540.3482314230874692No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCG2500.34274746366876885No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2460.3372635042500685No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCG2390.32766657526734305No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG2300.31532766657526734No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG2200.3016177680285166No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2070.2837948999177406No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG2020.2769399506443652No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG2000.2741979709350151No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2000.2741979709350151No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2000.2741979709350151No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1910.2618590622429394No Hit
CCTANGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1860.25500411296956405No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT1840.25226213326021385No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG1800.24677817384151354No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCACAATGGGCGAAAGCCTGAT1800.24677817384151354No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1730.23718124485878805No Hit
CCTANGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1730.23718124485878805No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1720.23581025500411298No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCG1710.23443926514943791No Hit
CCTANGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1650.22621332602138744No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1590.217987386893337No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1580.21661639703866192No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1530.20976144776528655No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCG1520.20839045791061148No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCG1490.20427748834658624No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1430.1960515492185358No Hit
CCTANGGGAGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG1430.1960515492185358No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1410.19330956950918562No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCG1400.19193857965451055No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC1360.18645462023581028No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1350.18508363038113518No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC1340.1837126405264601No Hit
CCTANGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1330.182341650671785No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG1320.18097066081710994No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG1290.17685769125308473No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC1290.17685769125308473No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1270.1741157115437346No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1260.1727447216890595No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1220.16726076227035921No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1200.16451878256100905No Hit
CCTANGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1180.1617768028516589No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1180.1617768028516589No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1180.1617768028516589No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1140.15629284343295857No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC1100.1508088840142583No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCG1090.14943789415958322No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT1080.14806690430490815No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG1070.14669591445023308No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1040.14258294488620785No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1030.14121195503153278No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCACAATGGGCGAAAGCCTGAT960.13161502604880723No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC960.13161502604880723No Hit
CCTANGGGTGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG930.12750205648478202No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC900.12338908692075677No Hit
CCTANGGGAGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG870.11927611735673155No Hit
CCTANGGGGGGCAGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT840.11516314779270634No Hit
CCTANGGGTGGCTGCAGTGGGGAATCTTCCACAATGGGCGAAAGCCTGAT830.11379215793803127No Hit
CCTANGGGAGGCTGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCG820.11242116808335618No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC810.11105017822868112No Hit
CCTANGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC800.10967918837400603No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT780.10693720866465586No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT760.10419522895530574No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)