FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122328
Sequences flagged as poor quality0
Sequence length301
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT36142.9543522333398733No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT30272.474494800863253No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT27852.2766660126872016No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT26562.171211823948728No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT24121.971748087110065No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT22541.8425871427637173No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT21431.7518474919887517No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT19421.5875351513962461No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT18711.5294944738735206No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT16491.3480151723235891No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC15841.2948793407886992No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT15721.2850696488130273No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC13701.119939833889216No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC13311.088058334968282No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC12481.0202079654698843No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT11540.9433653783271205No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC10140.828918971944281No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT8680.7095677195736054No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC7770.6351775554247596No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT7050.5763194035707279No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT6830.5583349682819959No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT6670.5452553789811No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT6530.5338107383428161No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG6470.52890589235498No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG6130.5011117650905761No Hit
CCTACGGGTGGCTGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA5890.4814923811392322No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5800.4741351121574783No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5430.4438885618991564No Hit
CCTACGGGGGGCAGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA5200.42508665227911846No Hit
CCTACGGGGGGCTGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA5160.4218167549538945No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG5140.4201818062912825No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG5120.41854685762867044No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT5060.4136420116408345No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4770.3899352560329606No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG4640.3793080897259826No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4470.3654110260937806No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4450.3637760774311687No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC4390.3588712314433327No Hit
CCTACGGGCGGCTGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA4300.35151396246157873No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4090.33434700150415275No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGACCAAGAGTCTGA4060.3318945785102348No Hit
CCTACGGGAGGCAGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA3590.29347328493885294No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3580.2926558106075469No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3540.28938591328232294No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA3530.2885684389510169No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3440.281211169969263No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA3420.27957622130665094No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3370.275488849650121No Hit
CCTANGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3360.27467137531881497No Hit
CCTACGGGTGGCTGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT3230.26404420901183706No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG3160.25832188869269507No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGACCAAGAGTCTGA3150.25750441436138904No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3150.25750441436138904No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCAAGAGTCTGA3130.25586946569877705No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA3080.2517820940422471No Hit
CCTACGGGAGGCTGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA3030.24769472238571708No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2980.24360735072918713No Hit
CCTANGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2970.2427898763978811No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2960.2419724020665751No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2860.23379765875351516No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA2840.23216271009090314No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCAAGAGTCTGA2840.23216271009090314No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2750.22480544110914918No Hit
CCTACGGGGGGCAGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT2750.22480544110914918No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2610.21336080047086523No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2590.2117258518082532No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA2580.21090837747694724No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAGAGTCTGA2560.20927342881433522No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2550.20845595448302923No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA2540.20763848015172326No Hit
CCTANGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2510.20518605715780527No Hit
CCTANGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2500.20436858282649925No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA2500.20436858282649925No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2490.20355110849519326No Hit
CCTACGGGGGGCTGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT2410.19701131384474527No Hit
CCTACGGGCGGCTGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT2380.19455889085082728No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2310.1888365705316853No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2300.18801909620037932No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA2260.1847491988751553No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2250.18393172454384932No Hit
CCTACGGGAGGCAGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT2230.18229677588123733No Hit
CCTANGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2200.17984435288731934No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2170.17739192989340136No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2150.17575698123078937No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA2090.17085213524295337No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2000.16349486626119938No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA1970.1610424432672814No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGTCAATGGGCGGGAGCCTGAA1930.15777254594205742No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1860.15205022562291545No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1820.14878032829769144No Hit
CCTACGGGTGGCAGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA1810.14796285396638545No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA1810.14796285396638545No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGTCAATGGGCGGGAGCCTGAA1800.14714537963507945No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1700.1389706363220195No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1690.13815316199071348No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG1690.13815316199071348No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1680.13733568765940748No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA1680.13733568765940748No Hit
CCTANGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1640.1340657903341835No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1630.1332483160028775No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA1620.13243084167157151No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGTCAATGGGCGGGAGCCTGAA1610.13161336734026552No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1610.13161336734026552No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1600.1307958930089595No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGACCAAGAGTCTGA1570.12834347001504154No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGTCAATGGGCGGGAGCCTGAA1570.12834347001504154No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCACAATGGGCGAAAGCCTGAT1500.12262114969589955No Hit
CCTANGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1490.12180367536459356No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA1470.12016872670198156No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1400.1144464063828396No Hit
CCTACGGGAGGCTGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT1390.11362893205153358No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1390.11362893205153358No Hit
CCTACGGGTGGCTGNAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1380.11281145772022759No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1370.11199398338892158No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1350.11035903472630959No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCACAATGGGCGAAAGCCTGAT1350.11035903472630959No Hit
CCTANGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1350.11035903472630959No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1340.1095415603950036No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA1330.10872408606369759No Hit
CCTANGGGTGGCTGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA1290.10545418873847362No Hit
CCTANGGGGGGCAGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA1260.10300176574455562No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGACCAAGAGTCTGA1250.10218429141324963No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1230.10054934275063764No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGTCAATGGGCGGGAGCCTGAA1230.10054934275063764No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)