FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122328
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG42223.451376626773919No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG29352.399287162383101No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG29202.3870250474135113No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG25662.0976391341311884No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG23901.9537636518213326No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG18551.5164148845726244No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG18471.5098750899221764No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA17871.4608266300438166No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG15741.2867045974756393No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG14581.1918775750441435No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG13151.074978745667386No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG12621.0316526061081683No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA12230.9997711071872343No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA11660.9531750703027925No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG11110.9082139820809626No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA10670.8722451115034988No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG10210.8346412922634229No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA9880.8076646393303251No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG9390.7676083970963312No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG9250.7561637564580472No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG8150.6662415800143875No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA7810.6384474527499837No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG6870.5616048656072199No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6820.55751749395069No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA6520.53299326401151No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6260.5117389313975541No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA6220.5084690340723301No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG6160.5035641880844942No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG5720.4675953175070303No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG5430.4438885618991564No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5410.4422536132365444No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG4530.37031587208161665No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG4430.36214112876855664No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG4320.35314891112419067No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA4020.3286246811850108No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4020.3286246811850108No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG3850.3147276175528088No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3810.3114577202275849No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3750.30655287423974886No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG3610.29510823360146493No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCATCAG3440.281211169969263No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3370.275488849650121No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG3330.272218952324897No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG3260.266496632005755No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG3200.261591786017919No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG3080.2517820940422471No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG2810.22971028709698516No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2800.2288928127656792No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2770.22644038977176115No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG2750.22480544110914918No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATACTTTCGAGCCTCAG2700.22071806945261918No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCATCAG2610.21336080047086523No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG2590.2117258518082532No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2540.20763848015172326No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2530.20682100582041724No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCATCAG2470.20191615983258127No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2460.20109868550127527No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCATCAG2370.1937414165195213No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2320.1896540448629913No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2310.1888365705316853No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG2290.1872016218690733No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCATCAG2150.17575698123078937No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2150.17575698123078937No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG2150.17575698123078937No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2100.17166960957425936No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG2030.1659472892551174No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG2020.1651298149238114No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1970.1610424432672814No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1930.15777254594205742No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1870.15286769995422142No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1860.15205022562291545No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1850.15123275129160946No Hit
GACTACAAGGGTATCTAATCCCGTTCGATCCCCACGCTGTCGCGCCTCAG1840.15041527696030343No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1840.15041527696030343No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1810.14796285396638545No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATACTTTCGAGCCTCAG1720.1406055849846315No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCATCAG1710.13978811065332547No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATACTTTCGAGCCTCAG1700.1389706363220195No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG1680.13733568765940748No Hit
GACTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1670.13651821332810152No Hit
GANTACAAGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGCACCTCAG1650.1348832646654895No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1650.1348832646654895No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1630.1332483160028775No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1610.13161336734026552No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1610.13161336734026552No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1580.12916094434634753No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1580.12916094434634753No Hit
GACTANAAGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGCACCTCAG1570.12834347001504154No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1560.12752599568373554No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1560.12752599568373554No Hit
GANTANAAGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGCACCTCAG1540.12589104702112355No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1540.12589104702112355No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATACTTTCGAGCCTCAG1500.12262114969589955No Hit
GANTANAAGGGTATCTAATNCTGTTTGCTCCCCACGCTTTCGCACCTCAG1470.12016872670198156No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1470.12016872670198156No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1450.11853377803936957No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1410.11526388071414558No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCATCAG1400.1144464063828396No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG1390.11362893205153358No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATACTTTCGAGCCTCAG1360.1111765090576156No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1360.1111765090576156No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG1340.1095415603950036No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1260.10300176574455562No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG1260.10300176574455562No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG1260.10300176574455562No Hit
GACTACACGGGTATCTAATCCCGTTCGATCCCCACGCTGTCGCGCCTCAG1250.10218429141324963No Hit
GACTACAGGGGTATCTAATCCCGTTCGATCCCCACGCTGTCGCGCCTCAG1230.10054934275063764No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1230.10054934275063764No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)