FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004833321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17591
Sequences flagged as poor quality0
Sequence length35-251
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3912.22272753112387No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2611.48371326246376No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2551.449604911602524No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1941.102836677846626No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1901.080097777272469No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1871.063043601841851No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1831.0403047012676936No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1640.9322949235404469No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1580.898186572679211No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1430.8129156955261212No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1410.8015462452390426No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1370.7788073446648854No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1360.7731226195213462No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1320.7503837189471889No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1280.7276448183730317No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1220.6935364675117959No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1210.6878517423682564No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1150.6537433915070207No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1140.6480586663634813No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1060.6025808652151668No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1010.5741572394974702No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG970.551418338923313No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG970.551418338923313No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG950.5400488886362345No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA910.5173099880620772No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG910.5173099880620772No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA900.5116252629185379No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG890.5059405377749986No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG890.5059405377749986No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG880.5002558126314592No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG880.5002558126314592No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA840.47751691205730207No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG840.47751691205730207No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG830.4718321869137627No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA820.4661474617702234No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG820.4661474617702234No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG820.4661474617702234No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG790.4490932863396055No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG790.4490932863396055No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG780.4434085611960662No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG770.4377238360525269No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG750.4263543857654482No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG750.4263543857654482No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG750.4263543857654482No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG740.4206696606219089No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG720.40930021033483033No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG710.40361548519129103No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG700.39793076004775174No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG700.39793076004775174No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG680.3865613097606731No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA680.3865613097606731No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG680.3865613097606731No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG650.36950713433005516No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG640.36382240918651587No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG610.34676823375589794No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG610.34676823375589794No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA580.32971405832528No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG580.32971405832528No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG580.32971405832528No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG580.32971405832528No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG580.32971405832528No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA580.32971405832528No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG560.31834460803820136No Hit
GACTACAAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCACCCGTAGG560.31834460803820136No Hit
GACTACAAGGGTATCTAATCCGCCCCCGTGCTCATTGAATTCGTGCTGGT540.3069751577511228No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG540.3069751577511228No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG510.28992098232050484No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG510.28992098232050484No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG500.2842362571769655No Hit
GACTACAAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCCCCCGTAGG490.2785515320334262No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG460.26149735660280826No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG460.26149735660280826No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG460.26149735660280826No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG460.26149735660280826No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG460.26149735660280826No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG440.2501279063157296No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG440.2501279063157296No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG440.2501279063157296No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG430.24444318117219033No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCAG420.23875845602865103No Hit
GACTACAAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCGCCCGTAGG420.23875845602865103No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG410.2330737308851117No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG410.2330737308851117No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG410.2330737308851117No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG400.2273890057415724No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG390.2217042805980331No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG390.2217042805980331No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG380.2160195554544938No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG370.21033483031095446No Hit
GACTACAAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCCCCCGTAGG360.20465010516741516No Hit
GACTACACGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCACCCGTAGG350.19896538002387587No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA340.19328065488033655No Hit
GACTACACGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCCCCCGTAGG340.19328065488033655No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG330.18759592973679723No Hit
GACTACTAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCCCCCGTAGG330.18759592973679723No Hit
GACTACAGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCACCCGTAGG330.18759592973679723No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG320.18191120459325794No Hit
GACTACAAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCTCCCGTAGG320.18191120459325794No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG320.18191120459325794No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCAG320.18191120459325794No Hit
GACTACACGGGTATCTAATCCGCCCCCGTGCTCATTGAATTCGTGCTGGT310.17622647944971861No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG300.1705417543061793No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCAG300.1705417543061793No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG290.16485702916264No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG290.16485702916264No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCCCCCGTAGG290.16485702916264No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG290.16485702916264No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG290.16485702916264No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG290.16485702916264No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACATGAG280.15917230401910068No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCACCCGTAGG280.15917230401910068No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCAG280.15917230401910068No Hit
GACTACAGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCCCCCGTAGG280.15917230401910068No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG280.15917230401910068No Hit
GACTACTAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCACCCGTAGG270.1534875788755614No Hit
GACTACTAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCGCCCGTAGG270.1534875788755614No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG270.1534875788755614No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG270.1534875788755614No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG270.1534875788755614No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG270.1534875788755614No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG270.1534875788755614No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG270.1534875788755614No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACATGAG260.14780285373202207No Hit
GACTACAGGGGTATCTAATCCGCCCCCGTGCTCATTGAATTCGTGCTGGT260.14780285373202207No Hit
GACTACTCGGGTATCTAATCCGCCCCCGTGCTCATTGAATTCGTGCTGGT260.14780285373202207No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACATGAG260.14780285373202207No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG260.14780285373202207No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG250.14211812858848274No Hit
GACTACTAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCCCCCGTAGG250.14211812858848274No Hit
GACTACAGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCCCCCGTAGG250.14211812858848274No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCCCCCGTAGG250.14211812858848274No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG240.13643340344494345No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGTCTCAG240.13643340344494345No Hit
GACTACACGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCTCCCGTAGG240.13643340344494345No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACATGAG240.13643340344494345No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG230.13074867830140413No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG230.13074867830140413No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG230.13074867830140413No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG220.1250639531578648No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG220.1250639531578648No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG220.1250639531578648No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG220.1250639531578648No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG220.1250639531578648No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCGCCCGTAGG220.1250639531578648No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG220.1250639531578648No Hit
GACTACCCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCCCCCGTAGG220.1250639531578648No Hit
GACTACTAGGGTATCTAATCCGCCCCCGTGCTCATTGAATTCGTGCTGGT220.1250639531578648No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG220.1250639531578648No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACATGAG210.11937922801432552No Hit
GACTACAGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCTCCCGTAGG210.11937922801432552No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG210.11937922801432552No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG210.11937922801432552No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG210.11937922801432552No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCAG200.1136945028707862No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCAG200.1136945028707862No Hit
GACTACAGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCGCCCGTAGG200.1136945028707862No Hit
GACTACACGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCGCCCGTAGG190.1080097777272469No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG190.1080097777272469No Hit
GACTACACGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCCCCCGTAGG190.1080097777272469No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG190.1080097777272469No Hit
GACTACCCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCACCCGTAGG180.10232505258370758No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG180.10232505258370758No Hit
GACTACCAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCCCCCGTAGG180.10232505258370758No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG180.10232505258370758No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG180.10232505258370758No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)