FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115104
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA86817.5418751737559075No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA61755.364713650264108No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA60605.264804003336113No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA52274.541110647762024No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA47374.115408673894913No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA39963.4716430358632193No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA31542.7401306644425913No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA30602.6584653878231856No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA19011.6515499026966918No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG11811.0260286349735892No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG8480.7367250486516541No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG7860.682860717264387No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG7310.63507784264665No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG6550.5690505977203225No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG4910.42657075340561584No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3990.3466430358632193No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3710.3223172087850987No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC3310.2875660272449263No Hit
GANTACAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA3110.27019043647484015No Hit
GANTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA2890.25107728662774537No Hit
GACTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA2760.23978315262718933No Hit
GANTANAAGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2700.23457047539616346No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2680.23283291631915484No Hit
GACTANAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2580.22414512093411176No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC2570.22327634139560742No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC2510.21806366416458162No Hit
GACTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA2250.19547539616346954No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2220.19286905754795663No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC2180.1893939393939394No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTCTCGCTCCTCAG2060.1789685849318877No Hit
GANTACAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA2040.17723102585487907No Hit
GANTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1980.17201834862385323No Hit
GACTACTAGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1970.1711495690853489No Hit
GANTACACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1940.168543230469836No Hit
GACTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1930.16767445093133168No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1910.16593689185432303No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1900.16506811231581875No Hit
GACTANAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1860.1615929941618015No Hit
GACTACAAGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1840.15985543508479289No Hit
GACTACAAGGGTATCTAATCCATTATTTGAAGCGGGCTCGGAGGAAACGT1770.1537739783152627No Hit
GANTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1770.1537739783152627No Hit
GANTANTAGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1760.1529051987767584No Hit
GANTACTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1760.1529051987767584No Hit
GACTACAAGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1730.15029886016124547No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1710.14856130108423687No Hit
GACTACAAGGGTATCTAATCCATTCTGAGTTGGTTTTTGTACATGGTGTA1680.14595496246872394No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1670.14508618293021963No Hit
GACTACAGGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1670.14508618293021963No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1660.14421740339171532No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1650.143348623853211No Hit
GANTANACGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1650.143348623853211No Hit
GACTANTCGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1620.14074228523769808No Hit
GANTACTCGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1610.13987350569919377No Hit
GACTANACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1590.13813594662218515No Hit
GANTANTCGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1530.1329232693911593No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1530.1329232693911593No Hit
GACTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1520.132054489852655No Hit
GANTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1490.12944815123714207No Hit
GACTANCCGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1480.12857937169863776No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1460.12684181262162914No Hit
GANTANAGGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1420.1233666944676119No Hit
GACTACAAGGGTATCTAATCCCAGTCTTTATGAATTGGATATTTGCTAAT1420.1233666944676119No Hit
GACTACACGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1420.1233666944676119No Hit
GANTANTCGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1400.12162913539060327No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTCTCGCTCCTCAG1390.12076035585209896No Hit
GACTANTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1380.11989157631359466No Hit
GACTACAAGGGTATCTAATCCTATTCCTGAGAGCTCTACCCTCATGACCT1360.11815401723658606No Hit
GANTACCCGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1330.11554767862107312No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1320.11467889908256881No Hit
GACTANTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1310.11381011954406449No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTCTCGCTCCTCAG1310.11381011954406449No Hit
GANTANCCGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1290.11207256046705588No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTCTCGCTCCTCAG1280.11120378092855157No Hit
GANTANCCGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1260.10946622185154295No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1260.10946622185154295No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTCTCGCTCCTCAG1250.10859744231303864No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1250.10859744231303864No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1250.10859744231303864No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1230.10685988323603003No Hit
GACTACAGGGGTATCTAATCCATTCTGAGTTGGTTTTTGTACATGGTGTA1230.10685988323603003No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1220.10599110369752572No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1180.10251598554350848No Hit
GACTACAGGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1180.10251598554350848No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1170.10164720600500418No Hit
GACTACACGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1160.10077842646649986No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)