FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121115
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA67085.538537753374892No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA46033.800520166783635No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA43863.6213516079758907No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA39813.2869586756388554No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA35262.91128266523552No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA30292.5009288692564917No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA24372.0121372249514926No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA23751.9609462081492797No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA15461.2764727738100152No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG7110.5870453701027949No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA5660.46732444371052306No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG5180.42769268876687444No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4970.4103537959790282No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG4920.4062254881723982No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG4460.3682450563514016No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG4290.3542088098088593No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3800.31375139330388474No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3720.30714610081327665No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3680.30384345456797257No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3570.2947611773933865No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3510.28980720802543036No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3330.27494529992156214No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3330.27494529992156214No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3300.2724683152375841No Hit
GACTACAAGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT3080.25430376088841183No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG3050.2518267762044338No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3000.24769846839780377No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2970.2452214837138257No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2930.24191883746852166No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2870.23696486810056558No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2840.23448788341658755No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2650.2188003137513933No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2650.2188003137513933No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2650.2188003137513933No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2640.21797465219006731No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2530.20889237501548114No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTCTCGAGCCTCAA2400.198158774718243No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2390.19733311315691696No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2390.19733311315691696No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2380.19650745159559097No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2370.19568179003426495No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2350.19403046691161294No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2340.19320480535028692No Hit
GACTANAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2200.18164554349172274No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2180.17999422036907073No Hit
GANTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA2180.17999422036907073No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2180.17999422036907073No Hit
GACTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA2160.1783428972464187No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2140.17669157412376668No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2120.17504025100111464No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2110.17421458943978863No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2110.17421458943978863No Hit
GACTACAGGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT2100.1733889278784626No Hit
GANTACAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA2100.1733889278784626No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2050.16926062007183257No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2050.16926062007183257No Hit
GACTACACGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT2040.16843495851050655No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG2030.16760929694918053No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG2020.1667836353878545No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG2000.1651323122652025No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1980.16348098914255046No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCTTCAG1980.16348098914255046No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1980.16348098914255046No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1930.1593526813359204No Hit
GANTANAAGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1920.15852701977459438No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1920.15852701977459438No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1880.15522437352929033No Hit
GACTACAAGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1860.15357305040663832No Hit
GACTACTCGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1860.15357305040663832No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1840.15192172728398629No Hit
GACTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1810.14944474260000826No Hit
GACTACTAGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1790.14779341947735622No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1750.14449077323205217No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG1710.14118812698674812No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTCTCGAGCCTCAA1690.1395368038640961No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCTTCAG1670.13788548074144408No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1670.13788548074144408No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1640.13540849605746605No Hit
GACTACTAGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1630.13458283449614003No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1590.13128018825083598No Hit
GANTACACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1580.13045452668950996No Hit
GACTACCCGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1550.12797754200553194No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCTTCAG1550.12797754200553194No Hit
GACTACAAGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC1550.12797754200553194No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1540.12715188044420592No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1520.12550055732155388No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1510.12467489576022787No Hit
GACTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1490.12302357263757586No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTCTCGAGCCTCAA1490.12302357263757586No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1480.12219791107624986No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTCTCGAGCCTCAA1470.12137224951492384No Hit
GACTANACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1460.12054658795359784No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1440.1188952648309458No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG1420.11724394170829378No Hit
GANTACAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1400.11559261858564175No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1400.11559261858564175No Hit
GACTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1400.11559261858564175No Hit
GACTACAGGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1390.11476695702431575No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1380.11394129546298973No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1380.11394129546298973No Hit
GACTACAAGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG1360.1122899723403377No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1360.1122899723403377No Hit
GANTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1360.1122899723403377No Hit
GANTANAGGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1340.11063864921768568No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1340.11063864921768568No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1330.10981298765635966No Hit
GANTACTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1330.10981298765635966No Hit
GACTACAAGGGTATCTAATCCCGTTTGCTCCCCACGCTTTCGCATCTGAG1320.10898732609503366No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1310.10816166453370764No Hit
GANTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1300.10733600297238163No Hit
GANTANACGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1300.10733600297238163No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1280.1056846798497296No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG1280.1056846798497296No Hit
GANTACCCGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1260.10403335672707757No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1250.10320769516575157No Hit
GANTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1250.10320769516575157No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCTTCAG1230.10155637204309954No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)