FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134350
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA63184.7026423520655No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA42653.1745441012281357No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA41853.1149981391886863No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA37692.8053591365835504No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA32472.4168217342761444No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG31002.3074060290286567No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA28312.1071827316710086No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA24541.8265723855601042No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA22181.650911797543729No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG20421.519910681056941No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG19951.4849274283587643No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG17811.325641979903238No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG16031.1931522143654634No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG15701.1685895050241906No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA13661.016747301823595No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG13380.9959062151097878No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG11680.8693710457759584No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG11350.8448083364346856No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG11010.8195013025679196No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG10340.7696315593598809No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG9950.7406029028656494No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG9730.7242277633048009No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG8760.6520282843319687No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6980.5195385187941942No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG6870.51135094901377No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG6690.4979531075548939No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG6640.49423148492742836No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG6470.4815779679940454No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5990.44585039077037586No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA5900.4391514700409378No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG5750.4279866021585412No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5670.4220320059545962No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG4390.32675846669147746No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4150.3088946780796427No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4120.3066617045031634No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3830.2850762932638631No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG3810.2835876442128768No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3510.2612579084480834No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3370.25083736509117976No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3240.24116114625976925No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG3110.23148492742835874No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2860.21287681429103092No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2540.18905842947525123No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2470.18384815779679942No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2410.17938221064384072No Hit
GANTACAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA2240.16672869371045776No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2170.16151842203200595No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG2150.16002977298101972No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2140.1592854484555266No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2050.15258652772608855No Hit
GACTACAAGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA2010.14960922962411613No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1960.14588760699665054No Hit
GACTANAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1930.1436546334201712No Hit
GACTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1880.13993301079270562No Hit
GACTACAAGGGTATCTAATCCTGTTCGATCCCCGCGCTTTCGTGCCTCAG1850.13770003721622628No Hit
GACTACAAGGGTATCTAATCCCGTTTGCTCCCCACGCTTTCGTACATGAG1850.13770003721622628No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1820.13546706363974692No Hit
GANTANAAGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1780.13248976553777447No Hit
GANTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1770.13174544101228136No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1740.129512467435802No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1650.12281354670636398No Hit
GACTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1620.12058057312988463No Hit
GACTACAAGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC1600.11909192407889839No Hit
GACTACTAGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1570.11685895050241905No Hit
GANTANACGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1550.11537030145143282No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1480.110160029772981No Hit
GACTACAGGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1480.110160029772981No Hit
GACTACAAGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC1470.1094157052474879No Hit
GACTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1450.10792705619650168No Hit
GACTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1420.10569408262002233No Hit
GANTACAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1380.10271678451804987No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)