FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115978
Sequences flagged as poor quality0
Sequence length301
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC76666.609874286502612No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC70196.052009863939713No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC62445.383779682353549No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC60775.239786856127886No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC51084.4042835710220904No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC37993.275621238510752No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC19651.6942868475055612No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC15491.3355981306799567No Hit
CCTANGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC13051.1252134025418614No Hit
CCTANGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC12901.1122799151563227No Hit
CCTANGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC11440.9863939712704134No Hit
CCTANGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC10270.8855127696632119No Hit
CCTANGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC9180.7915294279949645No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC8530.7354843159909639No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC8340.7191018986359482No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC7620.6570211591853627No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC7210.6216696269982238No Hit
CCTANGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6860.5914914897653003No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6320.5449309351773612No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG5140.44318750107779065No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4310.3716222042111435No Hit
CCTACGGGTGGCTGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG4040.3483419269171739No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG4020.3466174619324355No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG3910.3371329045163738No Hit
CCTANGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3600.31040369725292727No Hit
CCTACGGGGGGCAGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG3480.3000569073444964No Hit
CCTACGGGGGGCTGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG3290.28367448998948075No Hit
CCTACGGGCGGCTGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG3280.28281225749711153No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG3220.27763886254289605No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG3040.2621186776802497No Hit
CCTANGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2780.23970063287864937No Hit
CCTACGGGAGGCAGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG2680.23107830795495699No Hit
CCTACGGGTGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2360.20348686819914122No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2330.2009001707220335No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG2240.1931400782907103No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2210.19055338081360257No Hit
CCTACGGGTGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC2160.18624221835175636No Hit
CCTACGGGGGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2110.18193105588991015No Hit
CCTACGGGGGGCAGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2100.1810688233975409No Hit
CCTACGGGCGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2090.1802065909051717No Hit
CCTACGGGGGGCAGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC2060.17761989342806395No Hit
CCTACGGGTGGCTGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1980.17072203348911No Hit
CCTACGGGGGGCAGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1910.1646864060425253No Hit
CCTACGGGCGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1910.1646864060425253No Hit
CCTACGGGGGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1840.15865077859594062No Hit
CCTACGGGAGGCTGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG1840.15865077859594062No Hit
CCTANGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1700.14657952370277122No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATAGGTAGGGTGCGAGCGTTGTCCG1650.14226836124092498No Hit
CCTACGGGGGGCTGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1630.14054389625618652No Hit
CCTACGGGCGGCTGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1620.13968166376381727No Hit
CCTACGGGAGGCAGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1560.13450826880960182No Hit
CCTANGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1540.13278380382486335No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1520.13105933884012486No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1460.1258859438859094No Hit
CCTANGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1380.11898808394695545No Hit
CCTACGGGAGGCAGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1370.11812585145458622No Hit
CCTANGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1300.11209022400800152No Hit
CCTACGGGAGGCAGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1280.11036575902326304No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)