FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79321
Sequences flagged as poor quality0
Sequence length301
%GC55

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT23772.9966843584927068No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT23082.909696045183495No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT20332.563003492139534No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT17772.2402642427604293No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT15872.0007312061118743No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT12581.585960842652009No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG11451.4435017208557632No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11141.4044200148762624No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT11061.3943344133331652No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG10921.3766846106327455No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT9801.235486189029387No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG9701.2228791871005156No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT9061.1421943747557393No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG8681.0942877674260285No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7941.0009959531523809No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA7470.9417430440866857No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC7410.934178842929363No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG7030.8862722355996521No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC6810.8585368313561351No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT6700.8446691292343769No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC6440.8118909242193113No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG6360.8018053226762144No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG6280.7917197211331174No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC6260.7891983207473431No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA6220.7841555199757946No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5730.7223812105243252No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA5590.7047314078239054No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG5510.6946458062808083No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT5310.6694318024230658No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA5130.6467391989510974No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC5000.6303500964435648No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4910.6190037947075806No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4860.6127002937431449No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT4810.6063967927787093No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4600.5799220887280796No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT4540.5723578875707568No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT4520.5698364871849825No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG4480.5647936864134341No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4420.5572294852561113No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG4390.5534473846774498No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAGAGCCTGAT4380.5521866844845627No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAGAGCCTGAT4360.5496652840987885No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA4310.5433617831343528No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG4160.5244512802410459No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4130.5206691796623846No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4110.5181477792766103No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3890.4904123750330934No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3800.4790660732971092No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAGAGCCTGAT3780.4765446729113349No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA3550.44754856847493096No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3540.4462878682820438No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3480.43872366712472105No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT3370.42485596500296263No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC3270.41224896307409137No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAGAGCCTGAT3120.3933384601807844No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG3040.3832528586376874No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAGAGCCTGAT2960.37316725709459037No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2890.36434235574438045No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2780.35047465362262203No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT2760.3479532532368477No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG2740.3454318528510735No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG2510.3164357484146695No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2490.31391434802889523No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT2300.2899610443640398No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2280.2874396439782655No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2260.28491824359249124No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2150.2710505414707329No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT2150.2710505414707329No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2130.26852914108495857No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2120.2672684408920714No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2060.2597042397347487No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG2040.2571828393489744No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG1960.24709723780587742No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT1960.24709723780587742No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA1950.24583653761299026No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAGAGCCTGAT1950.24583653761299026No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1940.24457583742010314No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC1900.2395330366485546No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG1820.22944743510545756No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT1780.22440463433390906No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1760.2218832339481348No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1730.21810113336947343No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1720.21684043317658627No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1670.21053693221215064No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1630.2054941314406021No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT1590.2004513306690536No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG1550.19540852989750507No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG1540.19414782970461794No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC1500.1891050289330694No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT1460.1840622281615209No Hit
CCTANGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA1430.18028012758285952No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1410.17775872719708527No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1390.17523732681131102No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1380.17397662661842386No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA1370.17271592642553674No Hit
CCTANGGGGGGCAGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC1350.17019452603976248No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT1350.17019452603976248No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG1330.16767312565398823No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1320.1664124254611011No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG1310.16515172526821398No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1270.16010892449666547No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG1260.15884822430377832No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1260.15884822430377832No Hit
CCTANGGGGGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC1240.15632682391800407No Hit
CCTANGGGTGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC1230.15506612372511694No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGTGCAAACCTGAT1190.15002332295356843No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAGAGCCTGAT1190.15002332295356843No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT1140.14371982198913275No Hit
CCTANGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA1120.14119842160335852No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1110.1399377214104714No Hit
CCTANGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA1110.1399377214104714No Hit
CCTANGGGCGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC1090.1374163210246971No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1080.13615562083181No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT1080.13615562083181No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1070.13489492063892286No Hit
CCTANGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT1070.13489492063892286No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG1070.13489492063892286No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGGTGCAAACCTGAT1050.1323735202531486No Hit
CCTANGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1050.1323735202531486No Hit
CCTANGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1040.13111282006026148No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAGAGCCTGAT1030.12985211986737435No Hit
CCTANGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA1030.12985211986737435No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1020.1285914196744872No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1020.1285914196744872No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT1010.12733071948160007No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1010.12733071948160007No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1000.12607001928871295No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT1000.12607001928871295No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGGTGCAAACCTGAT990.12480931909582581No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC980.12354861890293871No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG960.12102721851716444No Hit
CCTANGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT950.1197665183242773No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGTGCAAACCTGAT920.11598441774561591No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAGAGCCTGAT910.11472371755272878No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC910.11472371755272878No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAGAGCCTGAT900.11346301735984167No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG880.1109416169740674No Hit
CCTACGGGGGGCAGNAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT870.10968091678118026No Hit
CCTANGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT870.10968091678118026No Hit
CCTANGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA860.10842021658829314No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGGTGCAAACCTGAT850.107159516395406No Hit
CCTANGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT840.10589881620251887No Hit
CCTACGGGTGGCTGNAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT830.10463811600963176No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT830.10463811600963176No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC820.10337741581674463No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT810.10211671562385749No Hit
CCTANGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT810.10211671562385749No Hit
CCTANGGGAGGCTGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCG800.10085601543097036No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)