FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177553
Sequences flagged as poor quality0
Sequence length301
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC129207.276700478167082No Hit
CCTACGGGGGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC128347.228264236594144No Hit
CCTACGGGGGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC111506.279815041142644No Hit
CCTACGGGCGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC102615.779119474185173No Hit
CCTACGGGAGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC89465.03849554780826No Hit
CCTACGGGAGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC62843.539224907492411No Hit
CCTACGGGTGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC34671.952656389923009No Hit
CCTACGGGCGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC27641.5567182756698No Hit
CCTANGGGTGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC22611.2734225836792394No Hit
CCTANGGGGGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC22521.2683536746774202No Hit
CCTANGGGGGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC19301.0869993748345563No Hit
CCTANGGGCGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC17891.0075864671393895No Hit
CCTANGGGAGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC16010.9017025902125No Hit
CCTANGGGAGGCTGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC11000.6195333224445658No Hit
CCTANGGGTGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC5390.30357132799783726No Hit
CCTACGGGTGGCTGCAGTTGCAACGGAGGAATTCTGTGGTGTTCCCGGTT4950.27878999510005464No Hit
CCTANGGGCGGCAGCAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC4750.2675257528737898No Hit
CCTACGGGGGGCAGCAGTTGCAACGGAGGAATTCTGTGGTGTTCCCGGTT4410.24837654108913956No Hit
CCTACGGGGGGCTGCAGTTGCAACGGAGGAATTCTGTGGTGTTCCCGGTT4370.24612369264388662No Hit
CCTACGGGCGGCTGCAGTTGCAACGGAGGAATTCTGTGGTGTTCCCGGTT4240.23880193519681447No Hit
CCTACGGGGGGCAGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC4100.2309169656384291No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4070.22922732930448936No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAGGCCTGAT3810.21458381441034508No Hit
CCTACGGGTGGCTGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC3800.21402060229903183No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAGGCCTGAT3510.19768745107094782No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG3500.19712423895963457No Hit
CCTACGGGAGGCAGCAGTTGCAACGGAGGAATTCTGTGGTGTTCCCGGTT3490.19656102684832136No Hit
CCTACGGGGGGCTGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC3450.19430817840306838No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG3340.18811284517862273No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG3300.18585999673336975No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3290.1852967846220565No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3280.18473357251074327No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3200.18022787562023734No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG3200.18022787562023734No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG3150.17741181506367112No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3140.17684860295235788No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG3070.1729061181731652No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3050.17177969395053871No Hit
CCTACGGGTGGCTGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC3040.17121648183922547No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAGGCCTGAT3030.17065326972791223No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2980.167837209171346No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2970.1672739970600328No Hit
CCTACGGGCGGCTGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC2960.16671078494871955No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2910.16389472439215333No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2860.1610786638355871No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAGGCCTGAT2840.15995223961296065No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2810.15826260327902092No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2610.14699836105275607No Hit
CCTACGGGAGGCAGNAGTGATAAATATTAAGCAATAAACGAAAGTTTGAC2570.14474551260750312No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG2520.1419294520509369No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2500.14080302782831042No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2480.13967660360568393No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG2450.1379869672717442No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2430.13686054304911774No Hit
CCTACGGGGGGCAGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC2420.1362973309378045No Hit
CCTACGGGAGGCTGCAGTTGCAACGGAGGAATTCTGTGGTGTTCCCGGTT2410.13573411882649125No Hit
CCTACGGGCGGCTGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC2390.13460769460386476No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG2380.13404448249255152No Hit
CCTACGGGGGGCTGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC2330.1312284219359853No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2290.12897557349073235No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2280.1284123613794191No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2270.12784914926810587No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2250.12672272504547938No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG2230.1255963008228529No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2220.12503308871153965No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG2210.1244698766002264No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG2210.1244698766002264No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2170.12221702815497346No Hit
CCTACGGGAGGCAGCAGAGATGGGTTAGCAGTTAAGAGCACTGGCAACTC2170.12221702815497346No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAGGCCTGAT2120.11940096759840724No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2120.11940096759840724No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2080.11714811915315426No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2070.11658490704184103No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC2060.11602169493052777No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG1920.1081367253721424No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC1900.10701030114951592No Hit
CCTACGGGTGGCTGCAGGGGCAGGACTCCAGGGACCCAGACAGGATCAGT1880.10588387692688943No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG1840.10363102848163647No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1830.10306781637032324No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG1780.100251755813757No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)