FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200503
Sequences flagged as poor quality0
Sequence length301
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC190629.507089669481255No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC164508.204366019461055No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC160147.986912914021237No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC151807.570959038019382No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC122396.104148067609961No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC92974.636838351545862No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC50292.508191897378094No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC39561.9730378099080812No Hit
CCTANGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC34201.705710139000414No Hit
CCTANGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC30621.527159194625517No Hit
CCTANGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC29411.4668109704094203No Hit
CCTANGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC25181.2558415584804217No Hit
CCTANGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC21811.0877642728537729No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT21191.0568420422637068No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT18200.9077170915148403No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT17370.866321202176526No Hit
CCTANGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC16910.8433789020613157No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT15170.7565971581472596No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT13090.652858061974135No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT9570.47729959152730883No Hit
CCTANGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC8800.43889617611706555No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT8020.3999940150521439No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT6830.34064328214540435No Hit
CCTANGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6760.3371520625626549No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT6200.30922230590065986No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5950.2967536645336978No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT5940.2962549188790193No Hit
CCTACGGGTGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC5910.29475868191498383No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5460.272315127454452No Hit
CCTACGGGGGGCAGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC5380.2683251622170242No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5290.26383645132491784No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT5150.25685401215941905No Hit
CCTACGGGGGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC4890.2438866251377785No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4670.23291422073485182No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4500.22443554460531762No Hit
CCTACGGGCGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC4430.22094432502256822No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4270.21296439454771252No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4150.20697944669157067No Hit
CCTACGGGAGGCAGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC4100.20448571841817828No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3900.1945108053246086No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3540.1765559617561832No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3360.1675785399719705No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3200.15959860949711474No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2980.1486262050941881No Hit
CCTACGGGAGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2940.14663122247547417No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2700.13466132676319056No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2440.12169393974155No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2130.10623282444651701No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2120.10573407879183852No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2020.1007466222450537No Hit
CCTACGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACT2010.1002478765903752No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)