FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135069
Sequences flagged as poor quality0
Sequence length301
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC59864.4318089272890155No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC52493.886161887627805No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC52043.852845582628138No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC48143.5641042726310252No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC38512.851135345638155No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT31062.2995654073103378No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC29292.168521274311648No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT26261.9441914873138912No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT23691.7539183676491277No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT23471.737630396315957No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT21481.5902982919840971No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT19571.4488890863188446No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT18271.3526419829864735No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT18121.3415365479865846No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT15731.1645899503216874No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC15621.1564459646551022No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT14601.0809290066558574No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT14171.0490934263228424No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC12890.9543270476571233No Hit
CCTACGGGTGGCTGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA10370.7677557396589891No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT10140.7507274059924928No Hit
CCTANGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC10060.7448045073258852No Hit
CCTANGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC8870.6567013896600996No Hit
CCTACGGGGGGCAGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA8820.6529995779934701No Hit
CCTANGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC8610.6374519689936256No Hit
CCTACGGGGGGCTGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA8130.6019145769939808No Hit
CCTANGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC7940.5878476926607882No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT7800.5774826199942251No Hit
CCTACGGGCGGCTGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA7200.5330608799946693No Hit
CCTANGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6380.4723511686619431No Hit
CCTACGGGAGGCAGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA6280.4649475453286839No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6060.44865957399551337No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT6050.44791921166218746No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT5600.4146029066625206No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5310.39313239899606867No Hit
CCTANGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC5200.3849884133294834No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5120.379065514662876No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5120.379065514662876No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4600.3405666733299277No Hit
CCTACGGGAGGCTGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA4580.3390859486632758No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4510.3339034123299943No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4320.3198365279968017No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4240.3139136293301942No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3940.29170275933041634No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3930.29096239699709037No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3790.28059732433052736No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3570.2643093529973569No Hit
CCTACGGGTGGCTGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT3500.2591268166640754No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3400.2517231933308161No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3170.23469485966431972No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2920.21618580133117146No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2900.21470507666451963No Hit
CCTACGGGGGGCTGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT2880.21322435199786777No Hit
CCTACGGGTGGCAGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA2640.19545565599804546No Hit
CCTACGGGGGGCAGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT2510.18583094566480834No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2450.1813887716648528No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2400.17768695999822315No Hit
CCTANGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2400.17768695999822315No Hit
CCTACGGGCGGCTGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT2320.17176406133161568No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2290.1695429743316379No Hit
CCTANGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2160.15991826399840084No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2150.1591779016650749No Hit
CCTACGGGAGGCAGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT2140.15843753933174895No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2130.15769717699842303No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1930.14288993033190445No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1930.14288993033190445No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1900.14066884333192664No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1820.13474594466531922No Hit
CCTANGGGTGGCTGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA1780.1317844953320155No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1760.13030377066536364No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1730.12808268366538586No Hit
CCTACGGGCGGCAGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA1670.12364050966543028No Hit
CCTACGGGCGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1610.11919833566547468No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1610.11919833566547468No Hit
CCTACGGGGGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1590.11771761099882282No Hit
CCTACGGGGGGCAGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1590.11771761099882282No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1590.11771761099882282No Hit
CCTACGGGTGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1580.11697724866549689No Hit
CCTACGGGGGGCAGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1560.11549652399884504No Hit
CCTACGGGAGGCTGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT1540.11401579933219318No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1520.1125350746655413No Hit
CCTANGGGGGGCTGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA1520.1125350746655413No Hit
CCTACGGGTGGCTGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1490.11031398766556352No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1490.11031398766556352No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1450.10735253833225981No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1380.1021700019989783No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1370.10142963966565237No Hit
CCTANGGGGGGCAGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA1360.10068927733232645No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)