FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153313
Sequences flagged as poor quality0
Sequence length301
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC53863.5130745598872894No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC50073.265867865086457No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC46813.053230971933235No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC44802.9221266298356956No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC37422.4407584484029403No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT36992.412711250839785No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT31662.065056453138351No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT28831.8804667575482836No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT28581.8641602473371468No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC27071.7656689256618812No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT26831.75001467585919No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT25221.6450007500994697No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT23341.522375793311722No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT22381.4597587941009569No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT20691.3495267850736727No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT17981.172764214384951No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT17051.1121039963995225No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC14970.9764338314428652No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT13520.8818560722182724No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC11590.7559698133882972No Hit
CCTANGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC10320.6731327415157228No Hit
CCTACGGGTGGCTGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA9390.6124725235302942No Hit
CCTANGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC9390.6124725235302942No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT9230.6020363569951668No Hit
CCTACGGGGGGCAGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA8880.5792072426995754No Hit
CCTANGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC8520.5557258679955385No Hit
CCTACGGGGGGCTGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA8430.5498555243195293No Hit
CCTANGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC8180.5335490141083926No Hit
CCTACGGGCGGCTGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA7220.4709320148976277No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7210.47027975448918224No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT7210.47027975448918224No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT7030.45853906713716386No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT6980.45527776509493645No Hit
CCTANGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6250.4076627552784174No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6160.4017924116024082No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5960.3887472034334988No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5920.38613816179971694No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5660.36917939118013476No Hit
CCTACGGGAGGCAGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA5540.36135226627878914No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT5440.3548296621943345No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5280.344393495659207No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5080.33134828749029765No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT5020.32743472503962484No Hit
CCTANGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC4950.32286890218050657No Hit
CCTACGGGAGGCTGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA4910.3202598605467247No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4790.31243273564537904No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4740.3091714336031517No Hit
CCTACGGGTGGCTGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT4280.2791674548146602No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4170.2719925903217601No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4170.2719925903217601No Hit
CCTACGGGGGGCAGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT4150.2706880695048691No Hit
CCTACGGGGGGCTGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT4070.2654699862373054No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC4020.26220868419507803No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC3830.24981573643461413No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC3770.24590217398394132No Hit
CCTACGGGTGGCTGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA3730.24329313235015945No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3600.2348137470403684No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3470.2263343617305773No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC3370.21981175764612262No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC3320.2165504556038953No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC3300.21524593478700438No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC3290.2145936743785589No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3280.21394141397011343No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3260.2126368931532225No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC3150.20546202866032234No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3120.20350524743498596No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3030.19763490375897674No Hit
CCTACGGGGGGCAGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA3000.19567812253364036No Hit
CCTACGGGCGGCTGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT2960.19306908089985847No Hit
CCTACGGGGGGCTGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA2900.18915551844918566No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2860.1865464768154038No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2850.1858942164069583No Hit
CCTACGGGCGGCTGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA2840.18524195599851284No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2840.18524195599851284No Hit
CCTACGGGAGGCAGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT2640.1721967478296035No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2610.1702399666042671No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2530.16502188333670337No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC2510.16371736251981242No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2440.15915153966069415No Hit
CCTANGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2440.15915153966069415No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2430.1584992792522487No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2320.15132441475934852No Hit
CCTACGGGTGGCAGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA2310.15067215435090306No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2280.14871537312556665No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC2240.14610633149178479No Hit
CCTANGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2190.14284502944955743No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA2170.1415405086326665No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2140.1395837274073301No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2140.1395837274073301No Hit
CCTACGGGAGGCTGCAGTAAGGAATATTGGACAATGGACGCAAGTCTGAT2110.1376269461819937No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA2100.13697468577354824No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2100.13697468577354824No Hit
CCTACGGGAGGCAGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA2080.1356701649566573No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2070.13501790454821183No Hit
CCTANGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2020.13175660250598448No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1990.1297998212806481No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1970.12849530046375715No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1940.12653851923842074No Hit
CCTACGGGTGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1930.12588625882997528No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1920.12523399842152982No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1880.12262495678774794No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1830.1193636547455206No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1820.11871139433707514No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1820.11871139433707514No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1820.11871139433707514No Hit
CCTANGGGTGGCTGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA1820.11871139433707514No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1800.1174068735201842No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA1780.11610235270329326No Hit
CCTACGGGTGGCTGCAGTGAGGAGTATTGGTCAATGGGCGGGAGCCTGAA1770.1154500922948478No Hit
CCTANGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1750.11414557147795687No Hit
CCTACGGGCGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1750.11414557147795687No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA1740.1134933110695114No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1740.1134933110695114No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1730.11284105066106592No Hit
CCTANGGGGGGCTGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA1730.11284105066106592No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1720.11218879025262046No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1710.11153652984417499No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1690.11023200902728406No Hit
CCTACGGGGGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1680.1095797486188386No Hit
CCTACGGGGGGCAGCAGTGAGGAGTATTGGTCAATGGGCGGGAGCCTGAA1650.10762296739350219No Hit
CCTACGGGCGGCAGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA1650.10762296739350219No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1630.10631844657661126No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1620.10566618616816578No Hit
CCTACGGGGGGCAGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1600.10436166535127485No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1570.10240488412593844No Hit
CCTANGGGGGGCAGCAGTAAGGAATATTGGTCAATGGACGGAAGTCTGAA1570.10240488412593844No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)