FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107229
Sequences flagged as poor quality0
Sequence length301
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC76937.174365143757752No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC68816.4171073123875075No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC65526.110287328987494No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC59245.524624868272576No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC49944.6573221796342406No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC37073.4570871685831257No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC20261.8894142442809316No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC15181.4156618079064431No Hit
CCTANGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC13751.2823023622340972No Hit
CCTANGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC12831.1965046769064338No Hit
CCTANGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC11311.0547519794085556No Hit
CCTANGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC10150.9465722892128061No Hit
CCTANGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC9680.9027408630128044No Hit
CCTANGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC7110.6630668942170495No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5950.5548872040213002No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5450.5082580272127876No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5440.5073254436766174No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4660.4345839278553376No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4100.3823592498298035No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3220.30029189864682126No Hit
CCTANGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3210.299359315110651No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG3050.284437978531927No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG2900.2704492254893732No Hit
CCTACGGGTGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2780.2592582230553302No Hit
CCTANGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2660.24806722062128717No Hit
CCTACGGGGGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2430.22661779928937134No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG2370.22102229807234985No Hit
CCTACGGGTGGCTGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG2320.21635938039149855No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2270.2116964627106473No Hit
CCTACGGGGGGCAGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2230.2079661285659663No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG2210.2061009614936258No Hit
CCTACGGGGGGCAGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG2070.19304479198724225No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG1960.18278637308936949No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1900.17719087187234797No Hit
CCTACGGGTGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1880.17532570480000748No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG1830.17066278711915622No Hit
CCTACGGGCGGCTGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG1760.16413470236596445No Hit
CCTACGGGCGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1720.16040436822128343No Hit
CCTACGGGGGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1700.1585392011489429No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1690.15760661761277267No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1690.15760661761277267No Hit
CCTACGGGGGGCAGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1640.15294369993192142No Hit
CCTACGGGGGGCTGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG1620.1510785328595809No Hit
CCTACGGGAGGCAGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG1610.15014594932341063No Hit
CCTACGGGAGGCAGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1580.14734819871489988No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1560.14548303164255938No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG1550.14455044810638912No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1430.1333594456723461No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1400.13056169506383536No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG1380.12869652799149484No Hit
CCTACGGGAGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1300.12123585970213281No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG1300.12123585970213281No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG1300.12123585970213281No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG1290.12030327616596256No Hit
CCTACGGGCGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1260.1175055255574518No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG1210.11284260787660055No Hit
CCTACGGGAGGCAGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1160.10817969019574927No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCG1130.10538193958723853No Hit
CCTACGGGTGGCTGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT1100.10258418897872777No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)