FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004833368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49714
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG11712.355473307317858No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG11672.347427284064851No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG11342.2810475922275417No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG10522.1161041155408937No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG9721.9551836504807498No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG9211.8525968540049083No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG7991.6071931447881886No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG7521.5126523715653537No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG7301.4683992436738142No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG7221.4523071971677999No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG7211.450295691354548No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG6741.3557549181317134No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT6371.281329203041397No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG5961.198857464698073No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5951.1968459588848213No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5921.1908114414450657No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5031.0117874240656557No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG4860.9775918252403749No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG4760.9574767671078569No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC4740.9534537554813532No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG4560.917246650842821No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG4460.8971315927103028No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3930.7905217846079575No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC3850.7744297381019432No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC3720.7482801625296697No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3370.6778774590658567No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG3300.6637969183730941No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC3240.6517278834935832No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3030.6094862614152955No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3000.6034517439755401No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2980.5994287323490365No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2980.5994287323490365No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC2970.5974172265357847No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2950.5933942149092811No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG2870.5773021684032666No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA2740.5511525928309933No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG2630.5290260288852234No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA2570.5169569940057126No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2520.5068994649394537No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG2490.5008649474996983No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA2460.49483043005994287No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2420.4867844068069357No Hit
CCTACGGGTGGCTGCAGTGAGGAGTATTGGTCAATGGGCGGGAGCCTGAA2410.4847729009936839No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2380.47873838355392845No Hit
CCTACGGGGGGCAGCAGTGAGGAGTATTGGTCAATGGGCGGGAGCCTGAA2370.4767268777406766No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2340.47069236030092126No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2230.4485657963551514No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG2180.4385082672888925No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2160.4344852556623888No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA2140.4304622440358853No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG2120.42643923240938164No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2100.42241622078287805No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2070.4163817033431227No Hit
CCTACGGGGGGCAGCAGTGAAGAATATTGGTCAATGGGCGGGAGCCTGAA2050.41235869171661904No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2050.41235869171661904No Hit
CCTACGGGGGGCTGCAGTGAGGAGTATTGGTCAATGGGCGGGAGCCTGAA2010.40431266846361186No Hit
CCTACGGGTGGCTGCAGTGAAGAATATTGGTCAATGGGCGGGAGCCTGAA1980.39827815102385644No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1950.3922436335841011No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC1920.38620911614434567No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1890.38017459870459025No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1880.3781630928913384No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1860.37414008126483483No Hit
CCTACGGGCGGCTGCAGTGAAGAATATTGGTCAATGGGCGGGAGCCTGAA1830.3681055638250795No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA1810.3640825521985759No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1800.36207104638532406No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG1790.36005954057207223No Hit
CCTACGGGGGGCTGCAGTGAAGAATATTGGTCAATGGGCGGGAGCCTGAA1770.35603652894556864No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG1740.3500020115058133No Hit
CCTACGGGAGGCAGCAGTGAGGAGTATTGGTCAATGGGCGGGAGCCTGAA1630.32787544756004344No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1630.32787544756004344No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1630.32787544756004344No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1600.321840930120288No Hit
CCTACGGGCGGCTGCAGTGAGGAGTATTGGTCAATGGGCGGGAGCCTGAA1580.31781791849378443No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1570.31580641268053267No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1520.30574888361427366No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1420.28563382548175564No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1410.2836223196685039No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1380.2775878022287484No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1300.26149575572273404No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1300.26149575572273404No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1290.2594842499094822No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA1280.25747274409623044No Hit
CCTACGGGAGGCAGCAGTGAAGAATATTGGTCAATGGGCGGGAGCCTGAA1280.25747274409623044No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1270.2554612382829786No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1260.25344973246972685No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1230.24741521502997144No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1140.22931166271070524No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1120.22528865108420162No Hit
CCTACGGGAGGCTGCAGTGAGGAGTATTGGTCAATGGGCGGGAGCCTGAA1110.22327714527094986No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1090.21925413364444624No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1070.21523112201794264No Hit
CCTACGGGAGGCTGCAGTGAAGAATATTGGTCAATGGGCGGGAGCCTGAA1030.20718509876493543No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC1000.20115058132518004No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC970.19511606388542463No Hit
CCTANGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC960.19310455807217283No Hit
CCTANGGGAGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG960.19310455807217283No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC950.19109305225892104No Hit
CCTANGGGAGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG930.18707004063241742No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT910.18304702900591382No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT910.18304702900591382No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG900.18103552319266203No Hit
CCTANGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC820.16494347668664763No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT810.1629319708733958No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG790.15890895924689222No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA780.15689745343364042No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC780.15689745343364042No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC760.15287444180713683No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT720.14482841855412962No Hit
CCTANGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC710.14281691274087782No Hit
CCTANGGGCGGCTGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC690.1387939011143742No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT690.1387939011143742No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT630.12672486623486343No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC610.1227018546083598No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT600.12069034879510802No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT590.11867884298185621No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT580.11666733716860442No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT580.11666733716860442No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC570.11465583135535262No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA570.11465583135535262No Hit
CCTANGGGTGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG560.11264432554210081No Hit
CCTACGGGTGGCAGCAGTGAAGAATATTGGTCAATGGGCGGGAGCCTGAA560.11264432554210081No Hit
CCTANGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA560.11264432554210081No Hit
CCTANGGGCGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG560.11264432554210081No Hit
CCTACGGGTGGCAGCAGTGAGGAGTATTGGTCAATGGGCGGGAGCCTGAA560.11264432554210081No Hit
CCTANGGGTGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG540.1086213139155972No Hit
CCTANGGGAGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG520.10459830228909363No Hit
CCTACGGGCGGCAGCAGTGAAGAATATTGGTCAATGGGCGGGAGCCTGAA510.1025867964758418No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT510.1025867964758418No Hit
CCTANGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA510.1025867964758418No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT500.10057529066259002No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC500.10057529066259002No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)