FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004833369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49714
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG12842.5827734642153115No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG8841.7781711389145916No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG8331.6755843424387498No Hit
GACTACAAGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG7891.5870780866556706No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG7581.5247214064448646No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG7051.4181115983425192No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG6921.3919620227702458No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCGTACCTCAG6561.319547813493181No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG6201.247133604216116No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG5961.198857464698073No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5791.1646618658727923No Hit
GACTACAGGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG5351.0761556100897132No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG5301.0660980810234542No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG5181.0419600112644325No Hit
GACTACTAGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG5071.0198334473186628No Hit
GACTACACGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG5021.0097759182524038No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG4520.9092006275898137No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCGTACCTCAG4480.9011546043368065No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG4430.8910970752705475No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG4420.8890855694572958No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG4420.8890855694572958No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCGTACCTCAG4300.8649474996982742No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG4130.8307519008729934No Hit
GACTACTCGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG4090.8227058776199864No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG4000.8046023253007202No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3960.7965563020477129No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3930.7905217846079575No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3870.7784527497284467No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG3680.7402341392766626No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCGTACCTCAG3660.736211127650159No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3590.7221305869573963No Hit
GACTACCCGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG3520.7080500462646337No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3510.7060385404513819No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3460.6959810113851229No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG3310.6658084241863459No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCGTACCTCAG3280.6597739067465905No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG3210.6456933660538279No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG3030.6094862614152955No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG2960.5954057207225328No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2850.5732791567767631No Hit
GACTACCAGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG2790.5612101218972523No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2790.5612101218972523No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG2780.5591986160840005No Hit
GACTACTGGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG2780.5591986160840005No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2600.5229915114454681No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2590.5209800056322162No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2550.5129339823792091No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2490.5008649474996983No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCGTACCTCAG2490.5008649474996983No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2470.4968419358731947No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCGTACCTCAG2360.47471537192742486No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2340.47069236030092126No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2310.46465784286116585No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCGTACCTCAG2280.4586233254214105No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2220.4465542905418997No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2180.4385082672888925No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG2170.43649676147564065No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2150.4324737498491371No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2130.42845073822263346No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG2110.4244277265961298No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1930.38822062195759743No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1770.35603652894556864No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1710.34396749406605787No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1690.3399444824395542No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1690.3399444824395542No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG1660.33390996499979886No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1640.32988695337329527No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1630.32787544756004344No Hit
GACTACCGGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG1530.3077603894275254No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1510.3037373778010219No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG1470.29569135454801465No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1430.28764533129500747No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1400.28161081385525205No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1380.2775878022287484No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCGTACCTCAG1310.26350726153598586No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1280.25747274409623044No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1250.25143822665647503No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1220.2454037092167196No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG1180.23735768596371243No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1010.20316208713843184No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG850.17097799412640302No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA780.15689745343364042No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG770.15488594762038863No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG740.1488514301806332No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG710.14281691274087782No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG640.12873637204811522No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG630.12672486623486343No Hit
GANTACAAGGGTATCTAATCNTGTTCGCTCCCCACGCTTTCGTCCATCAG540.1086213139155972No Hit
GACTACAAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCC520.10459830228909363No Hit
GACTACAAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCG510.1025867964758418No Hit
GANTANAAGGGTATCTAATCNTGTTCGCTCCCCACGCTTTCGTCCATCAG510.1025867964758418No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG500.10057529066259002No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)