FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150945
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT104506.923051442578422No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT72214.78386167146974No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT69194.583788797244029No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT63904.233330020868528No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT54823.6317864122693693No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT45583.0196429162940146No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT37732.499585941899367No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT37312.471761237536851No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT22221.4720593593693068No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG11840.7843916658385506No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG8190.5425817350690648No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7930.5253569180827453No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG7650.5068071151744012No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG7600.5034946503693398No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG7130.4723574812017622No Hit
GACTACAAGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG5980.396170790685349No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5950.39418331180231214No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG5820.38557090330915234No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG5630.3729835370499189No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5610.37165855112789425No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5540.36702110040080826No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG5300.35112126933651333No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5160.34184636788234124No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG5040.3338964523501938No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4570.3027592831826162No Hit
GACTACAGGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG4480.2967968465335056No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4430.29348438172844415No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG4430.29348438172844415No Hit
GACTACACGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG4420.2928218887674318No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4340.28752194507933354No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4300.28487197323528435No Hit
GACTACTAGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG3930.26035973367782966No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG3840.25439729702871905No Hit
GACTACAAGGGTATCTAATCCTATCAGAGGTTTCCCTATCCAATTAATCA3750.24843486037960846No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG3740.24777236741859615No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3690.24445990261353473No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3640.2411474378084733No Hit
GANTACAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT3590.23783497300341183No Hit
GACTACTCGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG3430.2272350856272152No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3420.22657259266620294No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG3410.22591009970519063No Hit
GACTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT3310.21928517009506773No Hit
GANTANAAGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT3130.20736029679684653No Hit
GANTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT3100.20537281791380965No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3090.20471032495279737No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCTTCAG3080.2040478319917851No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG3070.2033853390307728No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3060.2027228460697605No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2780.18417304316141642No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG2740.18152307131736725No Hit
GACTANAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT2730.18086057835635497No Hit
GANTACACGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2710.1795355924343304No Hit
GANTACAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2610.1729106628242075No Hit
GACTACCCGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG2590.1715856769021829No Hit
GACTACTAGGGTATCTAATCCTAGTTTGGGTCTTAGCTGTCGTGTATTAT2570.17026069098015834No Hit
GACTACAAGGGTATCTAATCCTAGTTTGGGTCTTAGCTGTCGTGTATTAT2540.16827321209712148No Hit
GACTACACGGGTATCTAATCCTATCAGAGGTTTCCCTATCCAATTAATCA2520.1669482261750969No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG2520.1669482261750969No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2440.16164828248699858No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2420.160323296564974No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2390.1583358176819371No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2350.15568584583788797No Hit
GACTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2300.15237338103282652No Hit
GANTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2280.15104839511080195No Hit
GACTACAGGGGTATCTAATCCTATCAGAGGTTTCCCTATCCAATTAATCA2270.15038590214978964No Hit
GANTANACGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT2240.1483984232667528No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCTTCAG2220.14707343734472822No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCTTCAA2220.14707343734472822No Hit
GACTACTCGGGTATCTAATCCTATCAGAGGTTTCCCTATCCAATTAATCA2140.14177349365662992No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGAA2130.1411110006956176No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG2120.14044850773460532No Hit
GACTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2110.13978601477359304No Hit
GACTACTGGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG2100.13912352181258072No Hit
GANTACTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2040.135148564046507No Hit
GACTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2030.1344860710854947No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG2030.1344860710854947No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCTTCAG2020.13382357812448245No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2020.13382357812448245No Hit
GACTANAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT2020.13382357812448245No Hit
GACTACTAGGGTATCTAATCCTATCAGAGGTTTCCCTATCCAATTAATCA2020.13382357812448245No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCTTCAG2010.13316108516347014No Hit
GANTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1960.1298486203584087No Hit
GANTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1940.12852363443638412No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1930.1278611414753718No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1910.12653615555334724No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1890.12521116963132267No Hit
GANTANAGGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT1880.12454867667031039No Hit
GACTANTCGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1870.12388618370929808No Hit
GANTACTCGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1850.12256119778727352No Hit
GACTACCAGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG1820.12057371890423665No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG1810.11991122594322434No Hit
GACTACAGGGGTATCTAATCCTAGTTTGGGTCTTAGCTGTCGTGTATTAT1800.11924873298221207No Hit
GANTANTCGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1720.11394878929411376No Hit
GANTACCCGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1720.11394878929411376No Hit
GACTACACGGGTATCTAATCCTAGTTTGGGTCTTAGCTGTCGTGTATTAT1710.11328629633310147No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1690.11196131041107688No Hit
GACTANACGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT1690.11196131041107688No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGAA1670.11063632448905229No Hit
GACTANTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT1650.10931133856702774No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1650.10931133856702774No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1630.10798635264500314No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1630.10798635264500314No Hit
GACTACCCGGGTATCTAATCCTATCAGAGGTTTCCCTATCCAATTAATCA1630.10798635264500314No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGAA1600.10599887376196629No Hit
GACTACCGGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG1590.105336380800954No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCTTCAG1570.10401139487892941No Hit
GANTANTAGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT1560.10334890191791711No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGAA1550.10268640895690483No Hit
GACTACAAGGGTATCTAATCCAGGAGTTGTTTATTCTTGGCCTCAAAGTC1540.10202391599589256No Hit
GACTANTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT1540.10202391599589256No Hit
GANTANTCGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT1530.10136142303488024No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1520.10069893007386796No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA1520.10069893007386796No Hit
GACTACTCGGGTATCTAATCCTAGTTTGGGTCTTAGCTGTCGTGTATTAT1510.10003643711285568No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)