FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78771
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT25983.298168107552272No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT25243.2042249051046703No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT21692.75355143390334No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT21652.7484734229602266No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT20732.631679171268614No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT20122.554239504386132No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT20072.5478919907072397No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT18942.4044381815642812No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT16772.1289560879003697No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT15201.9296441583831612No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT11191.4205735613360246No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT10951.3901054956773433No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT7040.8937299259879905No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA7000.8886519150448768No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT6750.856914346650417No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA5970.7578931332597022No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA5660.718538548450572No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5490.6969570019423392No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCG5130.651254903454317No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT5100.6474463952469817No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA5030.638559876096533No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4930.625864848738749No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCG4830.6131698213809651No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4510.5725457338360564No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCG4470.5674677228929429No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4330.5496946845920453No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4270.5420776681773749No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA3960.5027230833682447No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3940.5001840778966878No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCG3850.4887585532746823No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG3840.48748905053890396No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3800.4824110395957903No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3670.46590750403067116No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3580.45448197940866564No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG3550.45067347120133044No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC3520.44686496299399525No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT3450.43797844384354645No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC3360.4265529192215409No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG3310.4202054055426489No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG3300.41893590280687054No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCG3300.41893590280687054No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAGCCCTGAT3270.41512739459953535No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT3260.413857891863757No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG3220.4087798809206434No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3150.39989336177019463No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC3120.3960848535628594No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA3110.394815350827081No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3100.39354584809130266No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3050.3871983344124107No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT3020.3833898262050755No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA3010.3821203234692971No Hit
CCTACGGGGGGCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCG2990.37958131799774025No Hit
CCTACGGGTGGCTGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCG2840.36053877696106434No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2810.35673026875372915No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2810.35673026875372915No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2800.3554607660179508No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2710.3440352413959452No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAGCCCTGAT2670.3389572304528316No Hit
CCTACGGGGGGCTGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCG2660.3376877277170532No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAGCCCTGAT2660.3376877277170532No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2610.3313402140381613No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCG2550.32372319762349083No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2500.3173756839445989No Hit
CCTACGGGCGGCTGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCG2480.3148366784730421No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2450.3110281702657069No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC2440.30975866752992853No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2390.30341115385103656No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGACGAAAGTCTGAC2350.29833314290792295No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAGCCCTGAT2330.2957941374363662No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2320.2945246347005878No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC2310.2932551319648094No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2290.2907161264932526No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2240.2843686128143606No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2160.27421259092813344No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2150.272943088192355No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2130.27040408272079824No Hit
CCTACGGGAGGCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCG2130.27040408272079824No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2100.2665955745134631No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGACGAAAGTCTGAC2050.2602480608345711No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAGCCCTGAT1970.2500920389483439No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1940.24628353074100875No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1910.24247502253367356No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGCGCAATGGACGAAAGTCTGAC1840.2335885033832248No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGACGAAAGTCTGAC1830.2323190006474464No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1810.22977999517588962No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1770.22470198423277604No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT1650.20946795140343527No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAGCCCTGAT1610.2043899404603217No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCG1600.20312043772454327No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1590.2018509349887649No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCG1480.18788640489520253No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1470.18661690215942417No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGACGAAAGTCTGAC1450.18407789668786737No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1430.18153889121631056No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCG1430.18153889121631056No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1390.17646088027319698No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1390.17646088027319698No Hit
CCTACGGGAGGCTGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCG1370.1739218748016402No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1370.1739218748016402No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCG1310.16630485838696984No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1300.16503535565119143No Hit
CCTANGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1270.16122684744385624No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1230.15614883650074265No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCG1220.15487933376496427No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1150.1459928146145155No Hit
CCTANGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1140.1447233118787371No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1140.1447233118787371No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1130.1434538091429587No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1070.13583679272828833No Hit
CCTANGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1060.13456728999250994No Hit
CCTANGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1050.13329778725673155No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1020.12948927904939633No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCG1020.12948927904939633No Hit
CCTACGGGTGGCTGNAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1010.12821977631361794No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1000.12695027357783956No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCG990.12568077084206117No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG970.12314176537050438No Hit
CCTACGGGGGGCAGCAGCCGCGGTAAAACGGAGGATCCGAGCGTTATCCG970.12314176537050438No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGACGAAAGTCTGAC960.12187226263472599No Hit
CCTANGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT950.12060275989894757No Hit
CCTANGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA950.12060275989894757No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC950.12060275989894757No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA940.1193332571631692No Hit
CCTACGGGGGGCTGNAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT930.11806375442739078No Hit
CCTACGGGGGGCAGNAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT930.11806375442739078No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC920.1167942516916124No Hit
CCTANGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA910.11552474895583399No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT900.1142552462200556No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCG890.1129857434842772No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCG890.1129857434842772No Hit
CCTACGGGTGGCTGNAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT870.11044673801272042No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT870.11044673801272042No Hit
CCTANGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA850.10790773254116363No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGAA850.10790773254116363No Hit
CCTACGGGTGGCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCG810.10282972159805005No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT810.10282972159805005No Hit
CCTACGGGGGGCAGNAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT810.10282972159805005No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG800.10156021886227164No Hit
CCTANGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT800.10156021886227164No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA800.10156021886227164No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCG790.10029071612649325No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG790.10029071612649325No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAGCCCTGAT790.10029071612649325No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)