FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78771
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG49356.2649960010663825No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG34064.323926318061216No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG32884.174124995239365No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG29523.7475720760178235No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG25803.2753170583082607No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG21232.6951543080575338No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG17322.198778738368181No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG17192.182275202803062No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG11161.4167650531286895No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG6390.8112122481623949No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGGGCCTCAA5840.741389597694583No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5490.6969570019423392No Hit
GACTACAAGGGTATCTAATCCTGCTTGCTCCCCATGCTTTCGTACCTCAG5060.6423683843038682No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4920.6245953460029706No Hit
GACTACAAGGGTATCTAATCCCGTTTGCTCCCCACGCTTTCGTACATGAG4820.6119003186451867No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG4510.5725457338360564No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG4310.5471556791204886No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG4260.5408081654415965No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGGGCCTCAA4220.5357301544984829No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGGGCCTCAA3960.5027230833682447No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG3920.4976450724251311No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3770.47860253138845515No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3760.4773330286526768No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3720.47225501770956313No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3640.46209899582333597No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3570.4532124766728872No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG3490.44305645478666006No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGGGCCTCAA3410.4329004329004329No Hit
GACTACAGGGGTATCTAATCCTGCTTGCTCCCCATGCTTTCGTACCTCAG3300.41893590280687054No Hit
GACTACAGGGGTATCTAATCCCGTTTGCTCCCCACGCTTTCGTACATGAG3190.4049713727133082No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3130.39735435629863786No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGGGCCTCAA3080.39100684261974583No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3070.3897373398839674No Hit
GACTACTAGGGTATCTAATCCCGTTTGCTCCCCACGCTTTCGTACATGAG3050.3871983344124107No Hit
GACTACACGGGTATCTAATCCCGTTTGCTCCCCACGCTTTCGTACATGAG3030.38465932894085386No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3010.3821203234692971No Hit
GACTACTAGGGTATCTAATCCTGCTTGCTCCCCATGCTTTCGTACCTCAG2970.3770423125261835No Hit
GACTACACGGGTATCTAATCCTGCTTGCTCCCCATGCTTTCGTACCTCAG2920.3706947988472915No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG2920.3706947988472915No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2770.35165225781061554No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2770.35165225781061554No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2690.34149623592438844No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2530.3211841921519341No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGGGCCTCAA2520.3199146894161557No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2500.3173756839445989No Hit
GACTACTCGGGTATCTAATCCCGTTTGCTCCCCACGCTTTCGTACATGAG2450.3110281702657069No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2450.3110281702657069No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2450.3110281702657069No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2400.304680656586815No Hit
GACTACCCGGGTATCTAATCCCGTTTGCTCCCCACGCTTTCGTACATGAG2360.29960264564370137No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG2340.29706364017214454No Hit
GACTACTCGGGTATCTAATCCTGCTTGCTCCCCATGCTTTCGTACCTCAG2310.2932551319648094No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGGGCCTCAA2250.285638115550139No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGTCTCAG2250.285638115550139No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG2210.28056010460702546No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2200.27929060187124705No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2190.27802109913546863No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2170.27548209366391185No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2150.272943088192355No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2130.27040408272079824No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2110.26786507724924147No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGGGCCTCAA2060.2615175635703495No Hit
GACTACCCGGGTATCTAATCCTGCTTGCTCCCCATGCTTTCGTACCTCAG2030.2577090553630143No Hit
GACTACTGGGGTATCTAATCCCGTTTGCTCCCCACGCTTTCGTACATGAG2010.25517004989145753No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1980.25136154168412234No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1950.24755303347678714No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1860.23612750885478156No Hit
GANTANAAGGGTATCTAATNCTGTTTGCTCCCCATGCTTTCGTACCTCAG1790.22724098970433282No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1780.2259714869685544No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1760.22343248149699763No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1760.22343248149699763No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1760.22343248149699763No Hit
GANTACAAGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG1750.2221629787612192No Hit
GACTANAAGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG1750.2221629787612192No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGTCTCAG1730.21962397328966243No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1710.21708496781810563No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGTCTCAG1670.21200695687499205No Hit
GACTANAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1660.2107374541392137No Hit
GACTACTGGGGTATCTAATCCTGCTTGCTCCCCATGCTTTCGTACCTCAG1660.2107374541392137No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1660.2107374541392137No Hit
GACTACCAGGGTATCTAATCCTGCTTGCTCCCCATGCTTTCGTACCTCAG1660.2107374541392137No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1640.20819844866765685No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1630.2069289459318785No Hit
GACTACCAGGGTATCTAATCCCGTTTGCTCCCCACGCTTTCGTACATGAG1610.2043899404603217No Hit
GANTANAAGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG1610.2043899404603217No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1580.2005814322529865No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG1510.19169491310253775No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1450.18407789668786737No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1430.18153889121631056No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1390.17646088027319698No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGTCTCAG1380.1751913775374186No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCTCATGCTTTCGTACCTCAG1340.170113366594305No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGGGCCTCAA1330.1688438638585266No Hit
GACTANAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1330.1688438638585266No Hit
GACTANTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1320.1675743611227482No Hit
GACTANACGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG1320.1675743611227482No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGTCTCAG1290.16376585291541304No Hit
GACTACACGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG1280.16249635017963462No Hit
GACTANAGGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG1270.16122684744385624No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1260.15995734470807785No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1260.15995734470807785No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1260.15995734470807785No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1240.15741833923652104No Hit
GANTANACGGGTATCTAATNCTGTTTGCTCCCCATGCTTTCGTACCTCAG1240.15741833923652104No Hit
GANTACACGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG1220.15487933376496427No Hit
GANTACAGGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG1200.1523403282934075No Hit
GANTANAGGGGTATCTAATNCTGTTTGCTCCCCATGCTTTCGTACCTCAG1200.1523403282934075No Hit
GANTANAGGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG1190.15107082555762907No Hit
GACTANTAGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG1190.15107082555762907No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1180.14980132282185069No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1170.14853182008607227No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG1120.1421843064071803No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1110.1409148036714019No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1110.1409148036714019No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1100.13964530093562352No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1080.13710629546406672No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1070.13583679272828833No Hit
GACTANACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1060.13456728999250994No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1040.13202828452095314No Hit
GANTANACGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG1020.12948927904939633No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1000.12695027357783956No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGTCTCAG1000.12695027357783956No Hit
GACTACCGGGGTATCTAATCCCGTTTGCTCCCCACGCTTTCGTACATGAG990.12568077084206117No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGGGCTTCAA990.12568077084206117No Hit
GANTANTAGGGTATCTAATNCTGTTTGCTCCCCATGCTTTCGTACCTCAG990.12568077084206117No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG990.12568077084206117No Hit
GANTANTAGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG980.12441126810628278No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG960.12187226263472599No Hit
GANTANTCGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG940.1193332571631692No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG930.11806375442739078No Hit
GANTACTAGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG920.1167942516916124No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG920.1167942516916124No Hit
GANTACCCGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG870.11044673801272042No Hit
GACTANTCGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG870.11044673801272042No Hit
GANTANCCGGGTATCTAATNCTGTTTGCTCCCCATGCTTTCGTACCTCAG850.10790773254116363No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG850.10790773254116363No Hit
GANTACTGGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG830.10536872706960684No Hit
GACTANCCGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG820.10409922433382843No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCTCATGCTTTCGTACCTCAG810.10282972159805005No Hit
GANTANCCGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG810.10282972159805005No Hit
GACTANTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG800.10156021886227164No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG800.10156021886227164No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCTCATGCTTTCGTACCTCAG790.10029071612649325No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)