FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95398
Sequences flagged as poor quality0
Sequence length301
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC49835.223379945072224No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC45074.724417702677205No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC41274.326086500765215No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC37843.966540179039393No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC31653.3176796159248623No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC23032.4140967315876645No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC12341.2935281662089353No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC9280.9727667246692803No Hit
CCTANGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC8490.8899557642717876No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC8120.8511708840856202No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC7730.8102895238893897No Hit
CCTANGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC7640.8008553638441057No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC7250.7599740036478753No Hit
CCTANGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC7060.7400574435522758No Hit
CCTANGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6210.6509570431245938No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6130.6425711230843414No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5300.5555672026667225No Hit
CCTANGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC4870.5104928824503658No Hit
CCTANGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3780.3962347219019267No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3590.37631816180632716No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG2880.301893121449087No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG2680.28092832134845597No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCG2380.24948112119750937No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCG2330.24423992117235163No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG2230.23375752112203607No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2180.22851632109687833No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG2130.22327512107172057No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCG1920.201262080966058No Hit
CCTANGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1910.20021384096102643No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1860.1949726409358687No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1840.19287616092580556No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1830.19182792092077403No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1790.1876349609006478No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1760.18449024088555316No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCG1750.18344200088052162No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1740.18239376087549006No Hit
CCTANGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1710.1792490408603954No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1710.1792490408603954No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1690.1771525608503323No Hit
CCTANGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1680.17610432084530073No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCG1670.1750560808402692No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1670.1750560808402692No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1670.1750560808402692No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1610.1687666408100799No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1580.16562192079498522No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG1580.16562192079498522No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1550.16247720077989056No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1550.16247720077989056No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1530.16038072076982746No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1480.15513952074466972No Hit
CCTACGGGCGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1480.15513952074466972No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1460.1530430407346066No Hit
CCTACGGGTGGCTGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG1460.1530430407346066No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1430.14989832071951192No Hit
CCTACGGGGGGCAGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1430.14989832071951192No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1420.1488500807144804No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1400.1467536007044173No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1400.1467536007044173No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1390.14570536069938572No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG1390.14570536069938572No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1370.14360888068932265No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1350.14151240067925952No Hit
CCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1330.13941592066919642No Hit
CCTANGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1330.13941592066919642No Hit
CCTACGGGGGGCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCG1300.13627120065410175No Hit
CCTACGGGGGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1290.13522296064907022No Hit
CCTACGGGTGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1290.13522296064907022No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1260.13207824063397555No Hit
CCTACGGGTGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1260.13207824063397555No Hit
CCTACGGGGGGCAGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1260.13207824063397555No Hit
CCTANGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1250.13103000062894402No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1250.13103000062894402No Hit
CCTACGGGCGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1240.12998176062391245No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1240.12998176062391245No Hit
CCTACGGGTGGCTGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCG1220.12788528061384935No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1220.12788528061384935No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCG1210.12683704060881779No Hit
CCTACGGGTGGCTGCAGTCTGTTTAGCAAGCCCTCCAAAGGATTCTGAAA1200.12578880060378625No Hit
CCTACGGGTGGCTGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG1200.12578880060378625No Hit
CCTACGGGCGGCTGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCG1150.12054760057862848No Hit
CCTANGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1130.11845112056856537No Hit
CCTACGGGGGGCAGCAGGCAGCAGGCAGCGCGCGGTACTGGCGAGTTCTC1130.11845112056856537No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTATCCG1130.11845112056856537No Hit
CCTACGGGGGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1100.11530640055347073No Hit
CCTACGGGGGGCAGCAGCAACTGAAATTAATTTTCTCACGGTTCTGGAGG1090.11425816054843917No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1080.11320992054340762No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1080.11320992054340762No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTATCCG1080.11320992054340762No Hit
CCTACGGGGGGCAGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG1040.10901696052328141No Hit
CCTACGGGGGGCAGCAGTCTGTTTAGCAAGCCCTCCAAAGGATTCTGAAA1040.10901696052328141No Hit
CCTACGGGGGGCTGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG1030.10796872051824985No Hit
CCTACGGGTGGCTGCAGAGGTGGGTAATTCATTGCCTCAGCAATGTCTTC1030.10796872051824985No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1030.10796872051824985No Hit
CCTACGGGGGGCTGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCG1020.1069204805132183No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1020.1069204805132183No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1000.1048240005031552No Hit
CCTACGGGCGGCTGCAGGCATAAGCCATGATTGTACCTGGCAAATGGATT1000.1048240005031552No Hit
CCTACGGGAGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA990.10377576049812366No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT980.1027275204930921No Hit
CCTACGGGTGGCTGCAGGCATAAGCCATGATTGTACCTGGCAAATGGATT980.1027275204930921No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG980.1027275204930921No Hit
CCTACGGGTGGCTGCAGGACGCGAGCAAACACTGGCCAGGCAAATGGCAT970.10167928048806053No Hit
CCTACGGGTGGCTGCAGGTGAGAGAACATTTGACTGTGCCTGGGAAGGTT970.10167928048806053No Hit
CCTACGGGGGGCAGCAGGACGCGAGCAAACACTGGCCAGGCAAATGGCAT970.10167928048806053No Hit
CCTACGGGCGGCTGCAGGTCCTTTAGCTGAGGGTCTAGACACAATGGGTG970.10167928048806053No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC960.100631040483029No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)