FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67270
Sequences flagged as poor quality0
Sequence length301
%GC54

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG10371.5415489817154748No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG10361.5400624349635796No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT9911.473167831128289No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT9221.37059610524751No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGCTAATCG8851.3155938754273822No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT8611.2799167533818938No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG8491.2620781923591498No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGCTAATCG8411.250185818343987No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT8001.1892374015162777No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGCTAATCG7861.1684257469897428No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA7731.1491006392151033No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG7391.0985580496506615No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC7281.0822060353798129No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG6991.0390961795748477No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG6901.0257172588077896No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA6761.0049056042812545No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG6731.0004459640255685No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA6710.9974728705217779No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6620.9840939497547198No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC6610.9826074030028245No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC6470.9617957484762897No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA6390.9499033744611267No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGCTAATCG6310.938011000445964No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA6290.9350379069421733No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG5990.8904415043853129No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA5970.8874684108815222No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGCTAATCG5780.8592240225955107No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC5510.8190872602943362No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA5300.787869778504534No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA5110.7596253902185224No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG5090.7566522967147317No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG5040.7492195629552549No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA5020.7462464694514642No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4990.7417868291957782No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4960.7373271889400922No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG4950.7358406421881968No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC4410.6555671175858481No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA4360.6481343838263713No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4230.6288092760517318No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4230.6288092760517318No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC4190.6228630890441504No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA3920.5827263267429761No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC3830.5693474059759179No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGCTAATCG3770.5604281254645458No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA3620.5381299241861157No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC3600.5351568306823249No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA3570.5306971904266389No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3570.5306971904266389No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC3570.5306971904266389No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA3320.49353352162925523No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3230.48015460086219713No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC3230.48015460086219713No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG3160.46974877359892964No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG3100.4608294930875576No Hit
CCTACGGGCGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA3100.4608294930875576No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA3020.44893711907239486No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT2740.4073138100193251No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC2690.39988107625984837No Hit
CCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA2680.398394529507953No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT2590.3850156087408949No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2510.3731232347257321No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGCTAATCG2500.37163668797383675No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGGTACGAGCGTTGTCCG2340.3478519399435112No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2330.3463653931916159No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2320.3448788464397205No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGGTACGAGCGTTGTCCG2230.3314999256726624No Hit
CCTACGGGAGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA2100.31217481789802287No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2040.30325553738665084No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAC2030.3017689906347555No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT2030.3017689906347555No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG2020.30028244388286013No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1980.2943362568752787No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1970.2928497101233834No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAC1950.2898766166195927No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1920.28541697636390667No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC1910.28393042961201126No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1890.2809573361082206No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGCTAATCG1860.2764976958525346No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAC1830.2720380555968485No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1820.2705515088449532No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT1780.2646053218373718No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGGTACGAGCGTTGTCCG1760.2616322283335811No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1720.2556860413259997No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT1700.252712947822209No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1690.25122640107031363No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA1640.24379366731083693No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGGTACGAGCGTTGTCCG1630.2423071205589416No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1620.24082057380704625No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAC1590.23636093355136018No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1560.23190129329567413No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1560.23190129329567413No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGCTAATCG1550.2304147465437788No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1510.22446855953619743No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG1490.2214954660324067No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1480.22000891928051136No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGGTACGAGCGTTGTCCG1460.21703582577672068No Hit
CCTANGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1440.21406273227293No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1420.2110896387691393No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGCTAATCG1420.2110896387691393No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1410.20960309201724395No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1370.20365690500966255No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAC1360.2021703582577672No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGCTAATCG1340.1991972647539765No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA1310.19473762449829046No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG1280.19027798424260445No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1270.1887914374907091No Hit
CCTANGGGGGGCAGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA1270.1887914374907091No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT1250.18581834398691838No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1230.1828452504831277No Hit
CCTANGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1230.1828452504831277No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGCTAATCG1180.17541251672365096No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGCTAATCG1180.17541251672365096No Hit
CCTANGGGTGGCTGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA1170.1739259699717556No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG1150.17095287646796492No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1120.16649323621227888No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1100.16352014270848816No Hit
CCTANGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1070.15906050245280215No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1060.1575739557009068No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1040.1546008621971161No Hit
CCTACGGGTGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1030.15311431544522075No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1020.15162776869332542No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC1010.15014122194143006No Hit
CCTANGGGGGGCTGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA1000.1486546751895347No Hit
CCTANGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA970.14419503493384866No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC970.14419503493384866No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT960.14270848818195334No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGGTACGAGCGTTGTCCG960.14270848818195334No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT950.14122194143005798No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC940.13973539467816262No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG940.13973539467816262No Hit
CCTANGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC940.13973539467816262No Hit
CCTANGGGAGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG940.13973539467816262No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAC940.13973539467816262No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT930.1382488479262673No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT920.13676230117437194No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG890.1323026609186859No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC890.1323026609186859No Hit
CCTANGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA860.12784302066299985No Hit
CCTACGGGCGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA830.12338338040731381No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT830.12338338040731381No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC820.12189683365541847No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGACAATGGGCGCAAGCCTGATC810.12041028690352312No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT810.12041028690352312No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC780.11595064664783707No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC770.11446409989594172No Hit
CCTANGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA770.11446409989594172No Hit
CCTANGGGCGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC750.11149100639215104No Hit
CCTANGGGCGGCTGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA750.11149100639215104No Hit
CCTANGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGGAAGGCTGAA740.11000445964025568No Hit
CCTANGGGTGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA740.11000445964025568No Hit
CCTANGGGAGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGCTAATCG730.10851791288836034No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT730.10851791288836034No Hit
CCTANGGGCGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA730.10851791288836034No Hit
CCTANGGGGGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA720.107031366136465No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTAGGGTACGAGCGTTGTCCG710.10554481938456965No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG710.10554481938456965No Hit
CCTANGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC700.10405827263267431No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC690.10257172588077894No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)