FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99581
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA60566.0814814070957315No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA41174.13432281258473No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA39593.975658007049537No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA36253.6402526586397004No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA32803.2938010263001978No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA27352.746507867966781No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA22832.292606019220534No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA22072.2162862393428466No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA13421.3476466394191664No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA6760.6788443578594311No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4720.47398600134563823No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4220.42377561984715956No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4180.41975878932728133No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3210.32235064922023277No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3100.3113043652905675No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2760.277161305871602No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2640.26511081431196715No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2530.25406453038230187No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG2410.24201403882266698No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2340.23498458541287995No Hit
GACTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA2040.20485835651379278No Hit
GANTACAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA2010.20184573362388405No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2010.20184573362388405No Hit
GANTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1990.19983731836394494No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1870.18778682680431005No Hit
GACTANAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1850.1857784115443709No Hit
GANTANAAGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1840.18477420391440133No Hit
GACTACAAGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1740.17473212761470563No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1640.1646900513150099No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1540.1546479750153142No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1530.1536437673853446No Hit
GACTACAAGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1520.15263955975537502No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1510.15163535212540544No Hit
GACTACTAGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1490.1496269368654663No Hit
GACTACAAGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC1470.14761852160552716No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1460.14661431397555758No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1450.14561010634558802No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1440.14460589871561844No Hit
GACTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1430.14360169108564888No Hit
GACTACAAGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC1410.1415932758257097No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG1410.1415932758257097No Hit
GANTACACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1410.1415932758257097No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG1400.14058906819574016No Hit
GANTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1380.138580652935801No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG1370.13757644530583144No Hit
GANTACAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1360.13657223767586185No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1360.13657223767586185No Hit
GACTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1340.1345638224159227No Hit
GACTACAAGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG1330.13355961478595313No Hit
GANTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1310.13155119952601402No Hit
GANTANACGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1300.13054699189604443No Hit
GACTACAAGGGTATCTAATCCATTCTGAGTTGGTTTTTGTACATGGTGTA1290.12954278426607485No Hit
GACTACAAGGGTATCTAATCCCAGTAAAAATTTCTTTTTAATAGAAATTA1280.12853857663610527No Hit
GACTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1270.1275343690061357No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1240.12452174611622699No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1230.12351753848625742No Hit
GACTACAAGGGTATCTAATCCTGCTTGGAAGCCTCAAGTAGCAGTAGATT1220.12251333085628784No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1200.12050491559634871No Hit
GACTACAGGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1200.12050491559634871No Hit
GANTACTCGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1170.11749229270643997No Hit
GACTANAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1170.11749229270643997No Hit
GACTACAGGGGTATCTAATCCCAGTAAAAATTTCTTTTTAATAGAAATTA1170.11749229270643997No Hit
GANTANTAGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1160.11648808507647042No Hit
GANTACTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1160.11648808507647042No Hit
GACTANACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1160.11648808507647042No Hit
GANTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1150.11548387744650085No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1140.11447966981653127No Hit
GACTACAGGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC1130.1134754621865617No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG1100.11046283929665299No Hit
GACTANTCGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1100.11046283929665299No Hit
GACTACTAGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1070.10745021640674425No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1060.10644600877677468No Hit
GACTACAGGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1050.10544180114680513No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1040.10443759351683554No Hit
GACTACACGGGTATCTAATCCTTAATGTTGTGGACTTGTAATTTGTTGGC1030.10343338588686597No Hit
GANTANAGGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1020.10242917825689639No Hit
GACTACAAGGGTATCTAATCCCAAGGTGCAGCTTTACCACTGGTCTGCAA1020.10242917825689639No Hit
GACTACACGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1020.10242917825689639No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG1020.10242917825689639No Hit
GACTANCCGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1020.10242917825689639No Hit
GACTANTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1010.10142497062692682No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1010.10142497062692682No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)