FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004833388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55886
Sequences flagged as poor quality0
Sequence length301
%GC53

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT15572.786028701284758No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT14842.6554056472103924No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT13162.3547936871488386No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT13032.331532047382171No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT11582.072075296138568No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT10941.9575564542103567No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT10171.819775972515478No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT9991.7875675482231685No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT9501.699889059871882No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT8061.4422216655334072No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG7341.31338796836417No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG7091.2686540457359625No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6931.2400243352539098No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT6181.1058225673692876No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG6051.0825609276026196No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG5991.07182478617185No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG5601.0020398668718462No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG5320.951937873528254No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG4800.8588913144615825No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG4550.814157391833375No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG4500.8052106073077335No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG4310.771212826110296No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT4310.771212826110296No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG4270.7640553984897828No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG4200.7515299001538847No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG4170.7461618294384997No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT4140.7407937587231149No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG4050.7246895465769603No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4050.7246895465769603No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT3630.6495365565615718No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG3550.6352217013205455No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG3410.6101707046487492No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG3400.6083813477436208No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3330.5958558494077228No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG3280.5869090648820814No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG3270.5851197079769531No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3090.5529112836846438No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT3070.5493325698743872No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2940.5260709301077193No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG2920.5224922162974627No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG2830.506388004151308No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT2690.4813370074795119No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2680.47954765057438353No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2600.4652327953333572No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2570.45986472461797234No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2500.44733922628207423No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCG2440.43660308485130445No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2440.43660308485130445No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2430.4348137279461761No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCG2400.42944565723079126No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2400.42944565723079126No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCG2330.4169201588948932No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGCCGCAAGGCTGAA2290.40976273127438No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2230.3990265898436102No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT2190.391869162223097No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2180.3900798053179687No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2130.38113302079232725No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCG2080.3721862362666858No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCG2060.3686075224564292No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1970.3525033103102745No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1940.3471352395948896No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG1920.343556525784633No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGCCGCAAGGCTGAA1890.3381884550692481No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1860.33282038435386324No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1860.33282038435386324No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGCCGCAAGGCTGAA1820.32566295673335005No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCG1790.3202948860179652No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1750.31313745839745194No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1740.31134810149232367No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCG1680.3006119600615539No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGCCGCAAGGCTGAA1640.2934545324410407No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCG1600.2862971048205275No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG1590.28450774791539923No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1570.28092903410514264No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1480.26482482195898793No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCG1470.26303546505385966No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGCAAGGCTGAA1460.26124610814873134No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCG1420.25408868052821815No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1350.24156318219232006No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1300.2326163976666786No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG1240.22188025623590882No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1170.20935475790001074No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG1140.20398668718462584No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1130.20219733027949754No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1120.20040797337436927No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT1100.19682925956411268No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1070.1914611888487278No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCG1060.18967183194359946No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG1020.18251440432308627No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1010.18072504741795797No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCG1000.1789356905128297No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG1000.1789356905128297No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG990.17714633360770138No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG930.16641019217693162No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT900.16104212146154673No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT900.16104212146154673No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT860.15388469384103354No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG850.1520953369359052No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGCAAGGCTGAA850.1520953369359052No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCG820.14672726622052035No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT810.14493790931539205No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG800.14314855241026375No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG800.14314855241026375No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG780.13956983860000716No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC770.13778048169487886No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT770.13778048169487886No Hit
CCTANGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT770.13778048169487886No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT760.13599112478975056No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC740.13241241097949397No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG720.1288336971692374No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT720.1288336971692374No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT720.1288336971692374No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG710.12704434026410907No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC700.12525498335898078No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT700.12525498335898078No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT680.1216762695487242No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT680.1216762695487242No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG670.11988691264359588No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC670.11988691264359588No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT670.11988691264359588No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC660.11809755573846759No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGCCGCAAGGCTGAA640.114518841928211No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT630.11272948502308269No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG620.11094012811795441No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG610.10915077121282611No Hit
CCTANGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT610.10915077121282611No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG600.10736141430769781No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT600.10736141430769781No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT600.10736141430769781No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT590.10557205740256953No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT590.10557205740256953No Hit
CCTANGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT580.10378270049744122No Hit
CCTACGGGTGGCTGCAGCCGCGGTAGTACGGAGGGTGCAAGCGTTAATCG580.10378270049744122No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCG580.10378270049744122No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG570.10199334359231292No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT570.10199334359231292No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG570.10199334359231292No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG560.10020398668718464No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT560.10020398668718464No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)