FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55886
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA20703.7039687936155743No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA14662.6231972229180833No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA13492.4138424650180723No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG12572.2492216297462693No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA12002.1472282861539562No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA10721.9181906022975341No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA8951.6014744300898258No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG8541.5281107969795655No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG8041.4386429517231507No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG7971.4261174533872525No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6991.2507604766846794No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6831.2221307662026268No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6781.2131839816769854No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6701.198869126435959No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG6331.132662920946212No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5591.0002505099667178No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG5350.9573059442436388No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG5310.9501485166231257No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTGAG5070.9072039509000466No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG4750.849944529935941No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG4740.8481551730308127No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4580.81952546254876No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4480.8016318934974771No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG4370.7819489675410657No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG4330.7747915399205526No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG4290.7676341123000393No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4200.7515299001538847No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG3910.699638549905164No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTGAG3670.656693984182085No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG3560.6370110582256737No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTGAG3460.6191174891743907No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3440.6155387753641341No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTGAG3340.5976452063128511No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3320.5940664925025946No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG3190.5708048527359266No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3160.5654367820205418No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3020.5403857853487457No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG2890.5171241455820778No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2890.5171241455820778No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTGAG2810.5028092903410515No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2600.4652327953333572No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2580.46165408152310056No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2490.4455498693769459No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2350.4204988727051498No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2330.4169201588948932No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTGAG2330.4169201588948932No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG2310.41334144508463655No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2270.4061840174641234No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGTACATGAG2180.3900798053179687No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2160.3865010915077121No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2140.3829223776974556No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTGAG2020.36145009483591595No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1950.3489245965000179No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG1870.3346097412589915No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1800.32208424292309346No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1750.31313745839745194No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG1750.31313745839745194No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTGAG1710.3059800307769388No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGTACATGAG1620.2898758186307841No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1590.28450774791539923No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1580.2827183910102709No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG1560.2791396772000143No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGTACATGAG1540.2755609633897577No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1510.27019289267437285No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGTACATGAG1450.25945675124360307No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1420.25408868052821815No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGTACATGAG1410.2522993236230898No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1400.25050996671796155No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1390.24872060981283328No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG1370.24514189600257666No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG1350.24156318219232006No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG1300.2326163976666786No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1270.2272483269512937No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTGAG1220.21830154242565222No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG1210.21651218552052395No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1190.21293347171026733No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1150.20577604408975417No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1140.20398668718462584No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGTACATGAG1090.19503990265898435No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG970.1735676197974448No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG960.1717782628923165No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT890.15925276455641843No Hit
GANTACAAGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGAGCCTCAA870.15567405074616184No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG860.15388469384103354No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGTACATGAG840.15030598003077694No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT830.14851662312564864No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG790.14135919550513545No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGTACATGAG780.13956983860000716No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT760.13599112478975056No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT750.13420176788462226No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG750.13420176788462226No Hit
GACTANAAGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGAGCCTCAA720.1288336971692374No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG710.12704434026410907No Hit
GANTANAAGGGTATCTAATNCTGTTTGCTCCCCACGCTTTCGAGCCTCAA680.1216762695487242No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT640.114518841928211No Hit
GACTACACGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT590.10557205740256953No Hit
GACTACAGGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT590.10557205740256953No Hit
GANTANAAGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGAGCCTCAA590.10557205740256953No Hit
GACTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA580.10378270049744122No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG570.10199334359231292No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)