FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82368
Sequences flagged as poor quality0
Sequence length301
%GC53

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC12631.533362470862471No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG12501.5175796425796426No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC11661.4155982905982907No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11661.4155982905982907No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG11321.3743201243201244No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10991.3342560217560218No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG10231.2419871794871795No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC10191.2371309246309246No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10161.2334887334887334No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT9801.1897824397824397No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG9201.116938616938617No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT9191.1157245532245532No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT8781.065947940947941No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT8761.0635198135198136No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC8641.048951048951049No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG8591.0428807303807304No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8561.0392385392385393No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG8291.006458818958819No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8130.9870337995337995No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG7810.9481837606837606No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7330.8899087024087025No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG7230.8777680652680652No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT7050.8559149184149184No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC7010.8510586635586636No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT6970.8462024087024088No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT6800.8255633255633256No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT6700.8134226884226884No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG6450.7830710955710956No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT6400.777000777000777No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6260.760003885003885No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT6140.7454351204351204No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5910.717511655011655No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC5640.6847319347319347No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG5630.683517871017871No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC5460.6628787878787878No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG5240.6361693861693862No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5000.607031857031857No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4960.6021756021756022No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC4530.5499708624708625No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4500.5463286713286714No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC4410.5354020979020979No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4410.5354020979020979No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC4350.5281177156177156No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4340.5269036519036518No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4180.5074786324786325No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC3980.4831973581973582No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG3950.4795551670551671No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC3890.47227078477078477No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3760.4564879564879565No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3670.4455613830613831No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3630.4407051282051282No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC3610.4382770007770008No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3500.4249222999222999No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC3410.4139957264957265No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3390.4115675990675991No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3370.4091394716394716No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3350.4067113442113442No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC3260.3957847707847708No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC3260.3957847707847708No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3200.3885003885003885No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3120.3787878787878788No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG3100.37635975135975136No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3010.3654331779331779No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3000.3642191142191142No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2780.3375097125097125No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2740.33265345765345766No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2660.32294094794094796No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2660.32294094794094796No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2650.3217268842268842No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC2550.3095862470862471No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2420.2938034188034188No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGTAGGCGGCAAGCGTTGTCCG2400.2913752913752914No Hit
CCTACGGGTGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG2390.29016122766122765No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2300.27923465423465427No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2280.2768065268065268No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2260.2743783993783994No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGTAGGCGGCAAGCGTTGTCCG2210.2683080808080808No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2190.2658799533799534No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2170.2634518259518259No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGTAGGCGGCAAGCGTTGTCCG2160.26223776223776224No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2110.25616744366744365No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2080.25252525252525254No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2080.25252525252525254No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2070.2513111888111888No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2010.24402680652680656No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2010.24402680652680656No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1990.2415986790986791No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGTAGGCGGCAAGCGTTGTCCG1940.23552836052836054No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1910.2318861693861694No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1860.22581585081585082No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGTAGGCGGCAAGCGTTGTCCG1800.21853146853146854No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1780.2161033411033411No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1760.2136752136752137No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1760.2136752136752137No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1750.21246114996114995No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1710.2076048951048951No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1710.2076048951048951No Hit
CCTACGGGCGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG1700.2063908313908314No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1670.20274864024864026No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1660.20153457653457654No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1620.1966783216783217No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1590.19303613053613053No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1590.19303613053613053No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1590.19303613053613053No Hit
CCTANGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1570.1906080031080031No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1480.17968142968142967No Hit
CCTANGGGTGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG1460.17725330225330224No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1430.1736111111111111No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1400.16996891996891997No Hit
CCTANGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1400.16996891996891997No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1370.16632672882672883No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1340.16268453768453767No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1340.16268453768453767No Hit
CCTANGGGGGGCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG1340.16268453768453767No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1330.16147047397047398No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1290.15661421911421913No Hit
CCTANGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1280.1554001554001554No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1250.15175796425796426No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1240.15054390054390054No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1180.14325951825951824No Hit
CCTANGGGCGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG1180.14325951825951824No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1150.13961732711732713No Hit
CCTANGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1150.13961732711732713No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1140.1384032634032634No Hit
CCTANGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1140.1384032634032634No Hit
CCTANGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1130.1371891996891997No Hit
CCTANGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1120.13597513597513597No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1120.13597513597513597No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1090.13233294483294483No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1060.1286907536907537No Hit
CCTANGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1060.1286907536907537No Hit
CCTANGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1050.12747668997668998No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1050.12747668997668998No Hit
CCTANGGGGGGCTGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCG1030.12504856254856256No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACGTAGGCGGCAAGCGTTGTCCG1010.12262043512043512No Hit
CCTANGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT990.1201923076923077No Hit
CCTANGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC980.11897824397824397No Hit
CCTANGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT970.11776418026418027No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT940.11412198912198912No Hit
CCTANGGGAGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG930.11290792540792541No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC910.11047979797979797No Hit
CCTANGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC900.10926573426573427No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC890.10805167055167055No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC890.10805167055167055No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC890.10805167055167055No Hit
CCTANGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT880.10683760683760685No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT870.10562354312354312No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT860.1044094794094794No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC840.10198135198135197No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT840.10198135198135197No Hit
CCTACGGGGGGCAGCAGTAGGGAGTCTTCGGCAATGGACGAAAGTCTGAC840.10198135198135197No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)