FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004833393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179367
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT148798.295282855820748No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT99425.542825603371858No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT98535.493206665663138No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT88454.931230382400329No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT79134.411625326843845No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT65743.6651111965969214No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT54173.02006500638356No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT50722.8277219332430157No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT32111.7901843705921379No Hit
GACTACAAGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG6330.3529077255013464No Hit
GANTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT4680.2609175600863035No Hit
GACTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT4590.25589991470002843No Hit
GACTANAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT4560.25422736623793674No Hit
GANTACAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT4530.25255481777584504No Hit
GACTACAGGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG4350.24251952700329493No Hit
GACTACACGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG4250.2369443654629893No Hit
GACTACAAGGGTATCTAATCCTATCAGAGGTTTCCCTATCCAATTAATCA4100.22858162315253083No Hit
GANTANAAGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT4060.2263515585364086No Hit
GACTACAAGGGTATCTAATCCTAGTTTGGGTCTTAGCTGTCGTGTATTAT4030.22467901007431687No Hit
GACTACTAGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG3930.2191038485340113No Hit
GACTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT3660.20405091237518605No Hit
GACTACTAGGGTATCTAATCCTAGTTTGGGTCTTAGCTGTCGTGTATTAT3490.19457313775666651No Hit
GACTACAGGGGTATCTAATCCTAGTTTGGGTCTTAGCTGTCGTGTATTAT3480.19401562160263594No Hit
GACTACTCGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG3370.18788294390829974No Hit
GANTACAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT3370.18788294390829974No Hit
GACTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT3220.1795202015978413No Hit
GANTACACGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT3160.17617510467365793No Hit
GACTACAAGGGTATCTAATCCATCTAGGGCTACACACAGTGAGACCCTGT3160.17617510467365793No Hit
GACTACAAGGGTATCTAATCCTTCCACCCATCCTTGGAGGACCTCATTTA3110.1733875239035051No Hit
GACTACCCGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG3060.17059994313335228No Hit
GANTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT3050.17004242697932173No Hit
GANTANACGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT3020.16836987851723004No Hit
GANTANAGGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT3000.16725484620916892No Hit
GACTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2990.16669733005513834No Hit
GACTACAAGGGTATCTAATCCCTAAACAACCACCAAACCCAGTCACTATT2980.1661398139011078No Hit
GANTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2930.16335223313095498No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2930.16335223313095498No Hit
GACTANACGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT2920.1627947169769244No Hit
GACTANAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT2900.1616796846688633No Hit
GACTACACGGGTATCTAATCCTAGTTTGGGTCTTAGCTGTCGTGTATTAT2850.15889210389871047No Hit
GANTANTAGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT2840.1583345877446799No Hit
GACTANTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT2790.15554700697452709No Hit
GANTANTCGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2750.15331694235840482No Hit
GANTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2720.15164439389631315No Hit
GANTACTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2710.15108687774228258No Hit
GANTACTCGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2590.14439668389391583No Hit
GACTANTCGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2580.14383916773988525No Hit
GACTACAAGGGTATCTAATCCAGGAGTTGTTTATTCTTGGCCTCAAAGTC2570.1432816515858547No Hit
GACTACAAGGGTATCTAATCCTGTTCGATCCCCGCACTTTCGTGCCTCAG2510.13993655466167132No Hit
GACTANTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT2410.1343613931213657No Hit
GACTACACGGGTATCTAATCCTATCAGAGGTTTCCCTATCCAATTAATCA2400.13380387696733512No Hit
GACTACAGGGGTATCTAATCCTATCAGAGGTTTCCCTATCCAATTAATCA2400.13380387696733512No Hit
GANTANTCGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT2330.12990126388912118No Hit
GACTACCAGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG2320.12934374773509064No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2320.12934374773509064No Hit
GACTACAGGGGTATCTAATCCTTCCACCCATCCTTGGAGGACCTCATTTA2250.1254411346568767No Hit
GANTANCCGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2210.12321107004075443No Hit
GANTANCCGGGTATCTAATNCCAGTTTGGGTCTTAGCTGTCGTGTATTAT2180.12153852157866273No Hit
GACTACTAGGGTATCTAATCCTATCAGAGGTTTCCCTATCCAATTAATCA2140.11930845696254049No Hit
GACTACTCGGGTATCTAATCCTAGTTTGGGTCTTAGCTGTCGTGTATTAT2120.11819342465447936No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2090.11652087619238767No Hit
GACTANCCGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT2070.11540584388432655No Hit
GACTACACGGGTATCTAATCCTTCCACCCATCCTTGGAGGACCTCATTTA2070.11540584388432655No Hit
GACTACTCGGGTATCTAATCCTATCAGAGGTTTCCCTATCCAATTAATCA2070.11540584388432655No Hit
GACTACTGGGGTATCTAATCCCTAACTATTTATGTTTTGTTCTTTTATAG2020.11261826311417372No Hit
GACTACAGGGGTATCTAATCCATCTAGGGCTACACACAGTGAGACCCTGT2000.1115032308061126No Hit
GANTACCCGGGTATCTAATCNCAGTTTGGGTCTTAGCTGTCGTGTATTAT1990.11094571465208206No Hit
GACTANCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTGTCGTGTATTAT1980.11038819849805148No Hit
GACTACACGGGTATCTAATCCATCTAGGGCTACACACAGTGAGACCCTGT1970.10983068234402091No Hit
GACTACTAGGGTATCTAATCCATCTAGGGCTACACACAGTGAGACCCTGT1950.10871565003595979No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1940.10815813388192924No Hit
GACTACACGGGTATCTAATCCTGTTCGATCCCCGCACTTTCGTGCCTCAG1930.10760061772789867No Hit
GACTACAGGGGTATCTAATCCCTAAACAACCACCAAACCCAGTCACTATT1930.10760061772789867No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1930.10760061772789867No Hit
GACTACAGGGGTATCTAATCCAGGAGTTGTTTATTCTTGGCCTCAAAGTC1900.10592806926580697No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1880.10481303695774585No Hit
GACTACTAGGGTATCTAATCCTTCCACCCATCCTTGGAGGACCTCATTTA1850.10314048849565415No Hit
GACTACACGGGTATCTAATCCAGGAGTTGTTTATTCTTGGCCTCAAAGTC1820.10146794003356248No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)