FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83646
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG38114.556105492193291No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG26583.1776773545656694No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG24982.986395045788203No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG23792.8441288286349615No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG20212.4161346627453795No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG17602.1041053965521366No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG14351.7155632068479068No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG13481.611553451450159No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG8661.03531549625804No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG8160.9755397747650814No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTTAA7730.9241326542811371No Hit
GACTACAAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG6990.8356645864715586No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG6280.7507830619515578No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG5720.6838342538794443No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG5370.6419912488343734No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTTAA5100.6097123592281759No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG5100.6097123592281759No Hit
GACTACAGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG4870.582215527341415No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTTAA4780.5714558974726826No Hit
GACTACACGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG4750.567869354183105No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTTAA4640.5547186954546541No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4560.5451545800157808No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG4250.5080936326901465No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTTAA4090.4889654018123999No Hit
GACTACTAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG3990.4770102575138082No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTTAA3990.4770102575138082No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA3900.4662506276450757No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3900.4662506276450757No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3830.4578820266360615No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG3770.4507089400569065No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3730.4459268823374698No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3720.44473136790761064No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG3660.43755828132845564No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3620.43277622360901896No Hit
GACTACTCGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG3550.4244076226000048No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGTGCCTCAG3530.42201659374028644No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG3370.40288836286253976No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTTAA2910.347894699089018No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCTTCAA2820.33713506922028547No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG2820.33713506922028547No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTTAA2810.3359395547904263No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2800.33474404036056715No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG2780.33235301150084884No Hit
GACTACCCGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG2710.32398441049183463No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG2710.32398441049183463No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2700.32278889606197547No Hit
GACTACTGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG2630.3144202950529613No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGTGCCTCAG2600.3108337517633838No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG2580.3084427229036654No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2530.3024651507543696No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG2460.29409654974535543No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG2450.2929010353154962No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2440.2917055208856371No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2370.2833369198766229No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA2370.2833369198766229No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG2350.2809458910169046No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2320.27735934772732707No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG2310.2761638332974679No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA2300.27496831886760875No Hit
GACTACCAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG2270.2713817755780312No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGTGCCTCAG2210.26420868899887623No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2180.26062214570929876No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2180.26062214570929876No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA2130.25464457356000286No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGTGCCTCAG2130.25464457356000286No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2080.24866700141070705No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2020.241493914831552No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2020.241493914831552No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1960.23432082825239703No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1960.23432082825239703No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1890.22595222724338285No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGTGCCTCAG1890.22595222724338285No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCTTCAA1880.22475671281352366No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1850.22117016952394614No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA1840.219974655094087No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTTAA1830.21877914066422782No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCTTCAA1830.21877914066422782No Hit
GACTACAAGGGTATCTAATCCTGTTCGATCCCCGCGCTTTCGTGCCTCAG1820.21758362623436864No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1810.21638811180450948No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG1760.21041053965521364No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA1760.21041053965521364No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1760.21041053965521364No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCTTCAA1690.20204193864619946No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGTGCCTCAG1640.19606436649690362No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1560.18650025105803028No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG1560.18650025105803028No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCTTCAA1550.1853047366281711No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCTCCATGCTTTCGTACCTCAG1540.18410922219831194No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1530.18291370776845275No Hit
GACTACAAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG1470.17574062118929776No Hit
GACTACACGGGTATCTAATCCTGTTCGATCCCCGCGCTTTCGTGCCTCAG1470.17574062118929776No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1440.17215407789972026No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1390.16617650575042442No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1390.16617650575042442No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1390.16617650575042442No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1370.16378547689070605No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1360.1625899624608469No Hit
GANTACAAGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG1340.16019893360112858No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1340.16019893360112858No Hit
GACTANAAGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG1320.1578079047414102No Hit
GANTANAAGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG1310.15661239031155105No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA1310.15661239031155105No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG1300.1554168758816919No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG1300.1554168758816919No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCTCCATGCTTTCGTACCTCAG1290.1542213614518327No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGTGCCTCAG1290.1542213614518327No Hit
GACTACAGGGGTATCTAATCCTGTTCGATCCCCGCGCTTTCGTGCCTCAG1280.15302584702197355No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1270.1518303325921144No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1260.1506348181622552No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1250.14943930373239606No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA1240.14824378930253687No Hit
GACTACAAGGGTATCTAATCCTGTTCGATCCCCGCACTTTCGTGCCTCAG1220.14585276044281856No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1210.14465724601295937No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1190.14226621715324103No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1190.14226621715324103No Hit
GACTACTCGGGTATCTAATCCTGTTCGATCCCCGCGCTTTCGTGCCTCAG1180.14107070272338187No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1180.14107070272338187No Hit
GACTACCGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG1150.13748415943380438No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCTTCAA1120.13389761614422685No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCTCCATGCTTTCGTACCTCAG1100.13150658728450854No Hit
GACTANAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1090.13031107285464938No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1080.12911555842479017No Hit
GACTACTAGGGTATCTAATCCTGTTCGATCCCCGCGCTTTCGTGCCTCAG1080.12911555842479017No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCTTCAA1080.12911555842479017No Hit
GANTANAAGGGTATCTAATNCTGTTTGCTCCCCATGCTTTCGTACCTCAG1080.12911555842479017No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1040.12433350070535353No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGTGCCTCAG1040.12433350070535353No Hit
GACTACCCGGGTATCTAATCCTGTTCGATCCCCGCGCTTTCGTGCCTCAG1020.12194247184563517No Hit
GACTACAGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG1020.12194247184563517No Hit
GANTACAGGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG1010.120746957415776No Hit
GANTACACGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG1000.11955144298591684No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCTTCAA980.11716041412619851No Hit
GACTANAGGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG940.11237835640676183No Hit
GACTACACGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG920.1099873275470435No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG920.1099873275470435No Hit
GANTANACGGGTATCTAATNCTGTTTGCTCCCCATGCTTTCGTACCTCAG920.1099873275470435No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG920.1099873275470435No Hit
GANTANAGGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG910.10879181311718432No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCTCCATGCTTTCGTACCTCAG910.10879181311718432No Hit
GANTANTAGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG890.10640078425746599No Hit
GACTACTAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG880.10520526982760682No Hit
GACTACCAGGGTATCTAATCCTGTTCGATCCCCGCGCTTTCGTGCCTCAG870.10400975539774765No Hit
GACTANACGGGTATCTAATCNTGTTTGCTCCCCATGCTTTCGTACCTCAG870.10400975539774765No Hit
GANTANAGGGGTATCTAATNCTGTTTGCTCCCCATGCTTTCGTACCTCAG870.10400975539774765No Hit
GACTANAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG870.10400975539774765No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG860.10281424096788849No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG860.10281424096788849No Hit
GACTACTCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG840.10042321210817014No Hit
GACTACTAGGGTATCTAATCCTGTTCGATCCCCGCACTTTCGTGCCTCAG840.10042321210817014No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)