FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114794
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA64315.602209174695542No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA45003.9200655086502776No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA41933.6526299283934702No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA37583.2736902625572766No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA34733.0254194470094258No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA29032.5288778159137237No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA23022.0053312890917647No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA22391.9504503719706605No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA13831.2047667996585187No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA9020.7857553530672334No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA6250.5444535428680942No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA6210.5409690401937384No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA5420.4721501123752112No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4760.4146558182483405No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4480.3902642995278499No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3740.32580100005226753No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3680.3205742460407338No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG3650.31796086903496695No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3370.2935693503144764No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3260.28398696795999795No Hit
GACTACAAGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT3070.26743558025680786No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2920.25436869522797356No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG2780.24217293586772828No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2710.2360750561876056No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG2420.21081241179852608No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2400.20907016046134816No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2370.20645678345558133No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2320.20210115511263654No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2310.2012300294440476No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2300.20035890377545865No Hit
GACTACACGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT2250.1960032754325139No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2200.19164764708956913No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2040.17770963639214593No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG2040.17770963639214593No Hit
GANTACAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA2040.17770963639214593No Hit
GACTACAGGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT2030.17683851072355697No Hit
GACTACTAGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1980.17248288238061224No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1980.17248288238061224No Hit
GANTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1980.17248288238061224No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1970.17161175671202328No Hit
GACTACAAGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1950.16986950537484535No Hit
GANTANAAGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1950.16986950537484535No Hit
GACTANAAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1950.16986950537484535No Hit
GACTANAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1940.16899837970625642No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1940.16899837970625642No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1890.16464275136331166No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1880.16377162569472273No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1870.16290050002613377No Hit
GACTACAAGGGTATCTAATCCCAGTAAAAATTTCTTTTTAATAGAAATTA1820.158544871683189No Hit
GACTACTAGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1790.15593149467742218No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1730.15070474066588846No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1690.14722023799153267No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1640.1428646096485879No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1630.14199348397999897No Hit
GACTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1530.13328222729410943No Hit
GANTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1520.1324111016255205No Hit
GACTANACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1510.13153997595693157No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG1490.12979772461975364No Hit
GACTACTCGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1470.12805547328257574No Hit
GACTACAAGGGTATCTAATCCATTATTTGAAGCGGGCTCGGAGGAAACGT1460.12718434761398678No Hit
GANTANTAGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1450.12631322194539785No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1450.12631322194539785No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1430.12457097060821995No Hit
GACTACTGGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1430.12457097060821995No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1420.12369984493963099No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1410.12282871927104203No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1390.12108646793386414No Hit
GANTACACGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1380.12021534226527518No Hit
GANTANAGGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1370.11934421659668623No Hit
GACTACAGGGGTATCTAATCCCAGTAAAAATTTCTTTTTAATAGAAATTA1360.1184730909280973No Hit
GANTACTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1350.11760196525950833No Hit
GACTACAGGGGTATCTAATCCTAGTTTGGGTCTTAGCTATTGTGTGTTCA1350.11760196525950833No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1350.11760196525950833No Hit
GACTACCCGGGTATCTAATCCCAGCATGAAGGCCTCACCCTCATGGATTT1340.11673083959091939No Hit
GANTACAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1340.11673083959091939No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1300.11324633691656358No Hit
GACTACAAGGGTATCTAATCCTGATCTAGTGCTGTGGGGTTCATTCTGCC1300.11324633691656358No Hit
GACTACACGGGTATCTAATCCCAGTAAAAATTTCTTTTTAATAGAAATTA1290.11237521124797464No Hit
GANTANACGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1280.11150408557938568No Hit
GACTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1280.11150408557938568No Hit
GACTACAAGGGTATCTAATCCCATCACAGGGGCTCCACTCTCATGACCTT1270.11063295991079673No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1240.10801958290502989No Hit
GACTACAAGGGTATCTAATCCAGTAGGAGTTGTCTTCATATGAGGAAGAG1230.10714845723644092No Hit
GACTACAAGGGTATCTAATCCCTTTGTAAACACTGTCAGAAATTTATCTT1220.10627733156785198No Hit
GANTANTCGGGTATCTAATNCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1210.10540620589926304No Hit
GANTANTAGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1210.10540620589926304No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1190.10366395456208513No Hit
GANTANAGGGGTATCTAATCNCAGTTTGGGTCTTAGCTATTGTGTGTTCA1190.10366395456208513No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1180.10279282889349617No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)