FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004833400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004833400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102568
Sequences flagged as poor quality0
Sequence length301
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC80257.8240776850479685No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC69886.813041104438031No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC69776.802316511972545No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC61856.030145854457531No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC50014.875789719990641No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC38253.729233289135013No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC20121.9616254582325872No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC16501.608688869822947No Hit
CCTANGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC13541.3200998362062242No Hit
CCTANGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC12291.1982294672802434No Hit
CCTANGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC12201.1894548007175727No Hit
CCTANGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC10591.0324857655409094No Hit
CCTANGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC8950.8725918415100226No Hit
CCTANGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6510.634700881366508No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3890.3792605880976523No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3730.3636611808751267No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3550.3461118477497855No Hit
CCTANGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3410.33246236643007565No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3380.3295374775758521No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3350.32661258872162857No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3090.3012635519850246No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3010.29346384837376177No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2990.2915139224709461No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2720.26518992278293424No Hit
CCTANGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2690.2622650339287107No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2550.24861555260900087No Hit
CCTACGGGTGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2530.24666562670618516No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2420.23594103424069884No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAT2390.2330161453864753No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2260.2203416270181733No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2240.2183917011153576No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2210.21546681226113407No Hit
CCTACGGGTGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC2160.21059199750409485No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAT2120.20669214569846345No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAT2110.2057171827470556No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG2090.2037672568442399No Hit
CCTACGGGTGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG2090.2037672568442399No Hit
CCTACGGGGGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC2070.20181733094142423No Hit
CCTACGGGGGGCAGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA2050.19986740503860853No Hit
CCTACGGGGGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2010.19596755323297715No Hit
CCTACGGGTGGCTGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA2000.1949925902815693No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2000.1949925902815693No Hit
CCTACGGGGGGCAGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2000.1949925902815693No Hit
CCTACGGGGGGCAGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1930.18816784962171437No Hit
CCTACGGGAGGCAGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1920.18719288667030654No Hit
CCTACGGGCGGCTGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA1880.18329303486467513No Hit
CCTACGGGCGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1880.18329303486467513No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1870.1823180719132673No Hit
CCTACGGGGGGCTGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA1860.18134310896185946No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1820.17744325715622805No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAT1800.17549333125341238No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1720.1676936276421496No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1720.1676936276421496No Hit
CCTACGGGGGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1710.16671866469074176No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1540.15014429451680836No Hit
CCTACGGGCGGCTGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1540.15014429451680836No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1530.14916933156540052No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1490.14526947975976912No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1480.14429451680836128No Hit
CCTACGGGGGGCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCG1470.14331955385695344No Hit
CCTACGGGGGGCAGCAGCTGCAGGTAGCACTGGCTGGGGGAGTACCAGCT1470.14331955385695344No Hit
CCTACGGGTGGCTGCAGCTGCAGGTAGCACTGGCTGGGGGAGTACCAGCT1430.13941970205132204No Hit
CCTACGGGGGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1410.13746977614850636No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAT1400.1364948131970985No Hit
CCTACGGGAGGCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1360.13259496139146712No Hit
CCTACGGGGGGCTGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCG1360.13259496139146712No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1360.13259496139146712No Hit
CCTACGGGTGGCTGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCG1350.13161999844005928No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1270.1238202948287965No Hit
CCTACGGGGGGCAGCAGCAGACAGACAAGTATAAATGGCAAATGACTAAC1250.12187036892598081No Hit
CCTACGGGAGGCAGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA1230.11992044302316512No Hit
CCTACGGGTGGCTGCAGGGTAAACAGCTGCCGTGTCCCACCTCTACCCTC1220.11894548007175727No Hit
CCTACGGGGGGCAGCAGGGTAAACAGCTGCCGTGTCCCACCTCTACCCTC1220.11894548007175727No Hit
CCTACGGGTGGCTGCAGGTAGGATCAGGATGGGGAGGCCAGGCTGGAACA1220.11894548007175727No Hit
CCTACGGGGGGCAGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA1220.11894548007175727No Hit
CCTACGGGCGGCTGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG1210.11797051712034942No Hit
CCTACGGGTGGCTGCAGGTCCCGAGCCCTGCCCCACGGGGATGCAGCTAA1190.11602059121753373No Hit
CCTACGGGGGGCAGCAGGTAGGATCAGGATGGGGAGGCCAGGCTGGAACA1170.11407066531471804No Hit
CCTACGGGCGGCTGCAGGGTAAACAGCTGCCGTGTCCCACCTCTACCCTC1140.1111457764604945No Hit
CCTACGGGAGGCAGCAGGGAACAAACCCAAGAAACACCTGAAATGTGGAC1120.1091958505576788No Hit
CCTACGGGGGGCTGCAGGTAGGATCAGGATGGGGAGGCCAGGCTGGAACA1110.10822088760627097No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAT1110.10822088760627097No Hit
CCTACGGGAGGCTGNAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1100.10724592465486311No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1090.10627096170345526No Hit
CCTACGGGGGGCTGCAGCTGCAGGTAGCACTGGCTGGGGGAGTACCAGCT1090.10627096170345526No Hit
CCTACGGGAGGCTGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCG1080.10529599875204743No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1050.10237110989782389No Hit
CCTACGGGAGGCTGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA1050.10237110989782389No Hit
CCTACGGGGGGCAGCAGGCAATAATCCTGGAAGCAGGAATAATGGGCTCT1040.10139614694641604No Hit
CCTACGGGCGGCTGCAGCTGCAGGTAGCACTGGCTGGGGGAGTACCAGCT1040.10139614694641604No Hit
CCTACGGGGGGCAGCAGTGAGCTGAGATCGTGTCACTGCACTCCTGCCTG1030.10042118399500818No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)